Oldbring J, Hellsten S, Lindholm K, Mikulowski P, Tribukait B
Department of Urology, University of Lund, Malmo General Hospital, Sweden.
Cancer. 1989 Nov 15;64(10):2141-5. doi: 10.1002/1097-0142(19891115)64:10<2141::aid-cncr2820641028>3.0.co;2-a.
A prospective study comprised operative specimens from 11 patients with transitional cell carcinoma of the renal pelvis or ureter. DNA analysis of the primary tumor and of multiple biopsy specimens from preselected sites of the surrounding urothelium was performed with flow cytometry. All Grade 3 tumors and 50% of the Grade 2 tumors were aneuploid, and the remainder were Grade 2 and diploid. All invasive tumors were aneuploid. Carcinoma in situ was found in some of the preselected biopsy specimens, all of which were aneuploid, from two patients. Close correlation thus was observed between aneuploidy and tumor invasiveness, whereas diploidy was seen only in noninvasive tumors with lower malignancy grade. Aneuploidy was also associated with increased risk of carcinoma in situ. The study indicated that DNA analysis may be useful for defining the malignant potential of urothelial tumors of the upper urinary tract more fully than conventional grading and staging permit.
一项前瞻性研究纳入了11例肾盂或输尿管移行细胞癌患者的手术标本。采用流式细胞术对原发肿瘤以及周围尿路上皮预选部位的多个活检标本进行DNA分析。所有3级肿瘤和50%的2级肿瘤为非整倍体，其余为2级且为二倍体。所有浸润性肿瘤均为非整倍体。在两名患者的一些预选活检标本中发现了原位癌，所有原位癌均为非整倍体。因此，观察到非整倍体与肿瘤浸润性密切相关，而二倍体仅见于恶性程度较低的非浸润性肿瘤。非整倍体还与原位癌风险增加有关。该研究表明，DNA分析可能比传统的分级和分期更有助于更全面地确定上尿路尿路上皮肿瘤的恶性潜能。
