Mai Truong Tuyet, Hop Duong Van, Anh Trinh Thi Van, Lam Nguyen Thi
National Institute of Nutrition, Vietnam. Email:
Institute of Microbiology and Biotechnology, Vietnam.
Asia Pac J Clin Nutr. 2017 Jan;26(1):72-77. doi: 10.6133/apjcn.112015.09.
To demonstrate the gastrointestinal survival of Lactobacillus casei strain Shirota (LcS) in healthy Vietnamese adults, a fermented milk drink containing LcS was administered daily for 14 days. Twenty-six healthy Vietnamese adults took part in the study. Each participant consumed 65 mL of a fermented milk drink containing LcS daily for 14 days. The drink contained a dose of 10 8 CFU/mL LcS. Fecal samples were collected before, during and after consuming the fermented milk drink. LcS was confirmed by culture and ELISA. After 7 and 14 days of ingesting fermented milk drink, LcS was recovered from fecal samples at average of 5.0×10 7 CFU/g feces (n=26) and 5.4×10 7 CFU/g feces (n=26), respectively. LcS persisted in 8 voluteers until day 42 (after 14 days stopping fermented milk drink) at 0.0033×10 7 CFU/g feces (n=8). We confirmed survival of LcS after passage through the gastrointestinal tract of Vietnamese adults.
为证明干酪乳杆菌鼠李糖亚种（LcS）在健康越南成年人胃肠道中的存活情况，连续14天每日给予含LcS的发酵乳饮料。26名健康越南成年人参与了该研究。每位参与者连续14天每天饮用65毫升含LcS的发酵乳饮料。该饮料中LcS的剂量为10⁸CFU/mL。在饮用发酵乳饮料之前、期间和之后采集粪便样本。通过培养和酶联免疫吸附测定法（ELISA）确认LcS。在摄入发酵乳饮料7天和14天后，分别从粪便样本中检出LcS，平均含量为5.0×10⁷CFU/g粪便（n = 26）和5.4×10⁷CFU/g粪便（n = 26）。LcS在8名志愿者的粪便中持续存在至第42天（停止饮用发酵乳饮料14天后），含量为0.0033×10⁷CFU/g粪便（n = 8）。我们证实了LcS在通过越南成年人胃肠道后仍能存活。
