Ali Rehab F M, El-Anany Ayman M
Biochemistry Department, Faculty of Agriculture, Cairo University.
J Oleo Sci. 2017;66(1):41-49. doi: 10.5650/jos.ess16126.
The current investigation aimed to evaluate the influence of roselle seeds oil (RSO), coconut oil (CNO) and binary mixture of them on serum lipids of experimental rats. Fatty acid composition of native and blended oils was determined. Thirty five male Albino rats (145- 160 g) were used throughout this study. The rats were fed AIN-93G diet containing 10% fat from CNO, RSO, B1 (25%RSO+ 75 %CNO), B2 (50 %RSO+ 50 %CNO or B3 (75 %RSO+ 25 % CNO) for eight weeks. Blood samples were collected at the beginning, every two weeks during the experiment, and at the end of the experiment. At the time of sacrifice, organs weights in relation to their body weights were immediately recorded. Substitution of 25, 50 and 75 % of CNO with equal amounts of RSO reduced saturated fatty acids by 16.04, 32.58 and 48.77 %, respectively in blended oils. The content of linoleic (C18:2) increased from not detected level in CNO to 9.81, 19.67 and 29.48 % in CNO blended with 25, 50 and 75 % of RSO, respectively. The relative liver weights of rats fed CNO was significantly higher than that of those fed RSO and blended oils. Mixing CNO with various levels of RSO attenuates the adverse effect in the relative liver weights which caused by CNO administration. At the end of the experiment, blinding coconut oil with 25, 50 and 75 % of roselle oil inhibited the elevation in total cholesterol by 9.69, 28.16 and 36.16 %, respectively compared to CNO rats. Rats fed diet containing CNO for 8 weeks had significantly the highest content (126.49 mg/dl) of low-density lipoprotein cholesterol, while those fed 100 % RSO (as a source of lipids) had the lowest concentration of LDL-C (64.32 mg/dL). Atherogenic index (AI) values of rats submitted B1, B2 and B3 were about 1.12, 1.23 and 1.28 times as low as those of rats fed CNO diet, respectively. The results of this study indicate that roselle seeds oil (RSO) reduces hyperlipidemia and hypercholesterolemia in rats fed diet rich in saturated fatty acids.
本研究旨在评估玫瑰茄籽油（RSO）、椰子油（CNO）及其二元混合物对实验大鼠血脂的影响。测定了天然油和混合油的脂肪酸组成。本研究共使用了35只雄性白化大鼠（体重145 - 160克）。给大鼠喂食含有10%来自CNO、RSO、B1（25%RSO + 75%CNO）、B2（50%RSO + 50%CNO）或B3（75%RSO + 25%CNO）脂肪的AIN - 93G饮食，持续8周。在实验开始时、实验期间每两周以及实验结束时采集血样。处死大鼠时，立即记录各器官重量与其体重的关系。用等量的RSO替代25%、50%和75%的CNO后，混合油中的饱和脂肪酸分别降低了16.04%、32.58%和48.77%。亚油酸（C18:2）的含量从CNO中未检测到的水平分别增加到与25%、50%和75%RSO混合的CNO中的9.81%、19.67%和29.48%。喂食CNO的大鼠相对肝脏重量显著高于喂食RSO和混合油的大鼠。将CNO与不同水平的RSO混合可减轻CNO给药引起的相对肝脏重量的不良影响。实验结束时，与喂食CNO的大鼠相比，用25%、50%和75%玫瑰茄油与椰子油混合分别使总胆固醇升高抑制了9.69%、28.16%和36.16%。喂食含CNO饮食8周的大鼠低密度脂蛋白胆固醇含量最高（126.49毫克/分升），而喂食100%RSO（作为脂质来源）的大鼠LDL - C浓度最低（64.32毫克/分升）。接受B1、B2和B3处理的大鼠的动脉粥样硬化指数（AI）值分别约为喂食CNO饮食大鼠的1.12、1.23和1.28倍。本研究结果表明，玫瑰茄籽油（RSO）可降低喂食富含饱和脂肪酸饮食大鼠的高脂血症和高胆固醇血症。
