Herrick Jeffrey E, Panza Gino S, Gollie Jared M
Department of Rehabilitation Science, George Mason University, 4400 University Drive, MS 2G7, Fairfax, VA 22030, USA.
J Obes. 2016;2016:8375828. doi: 10.1155/2016/8375828. Epub 2016 Dec 6.
Leptin (LEP) is associated with appetite regulation and metabolism. Concentration is linear with adiposity, suggesting LEP resistance. LEP circulates freely and bound with its soluble receptor (sOB-r); the ratio is the free leptin index (FLI), an index of leptin resistance; lower FLI suggests reduced biological action. . The aim was to determine the effect of changes in adipose tissue distribution on LEP, sOB-r, and FLI following 6 months (6 M) of a diet/exercise weight loss program (WLP). In addition, we aim to identify predictors of the FLI. . 6 M WLP consisted of diet/lifestyle interventions following ADA guidelines. Body composition was assessed by DXA. LEP and sOB-r analysis were done via ELISA. . 10 adults completed the WLP. Significant reductions were seen in total fat percentage (% fat), nontrunk fat, (NTF), and trunk fat (TF) from base to 3 m and 6 M ( ≤ 0.05). The FLI were reduced at 3 M and 6 M for males and 6 M for females. Total body fat and body weight predicted the FLI in both sexes. . LEP and FLI reductions following 6 M of WLP were achieved independent of sOB-r changes. We also demonstrate that the FLI can be predicted noninvasively through total fat mass and body weight in kilograms.
瘦素（LEP）与食欲调节和新陈代谢相关。其浓度与肥胖程度呈线性关系，提示存在瘦素抵抗。瘦素可自由循环，并与其可溶性受体（sOB-r）结合；二者的比例即游离瘦素指数（FLI），是瘦素抵抗的一个指标；较低的FLI表明生物活性降低。本研究旨在确定在进行为期6个月（6M）的饮食/运动减肥计划（WLP）后，脂肪组织分布变化对LEP、sOB-r和FLI的影响。此外，我们旨在确定FLI的预测因素。6M WLP包括遵循美国糖尿病协会指南的饮食/生活方式干预。通过双能X线吸收法（DXA）评估身体成分。通过酶联免疫吸附测定（ELISA）进行LEP和sOB-r分析。10名成年人完成了WLP。从基线到3个月和6个月时，总脂肪百分比（%脂肪）、非躯干脂肪（NTF）和躯干脂肪（TF）均显著降低（P≤0.05）。男性在3个月和6个月时以及女性在6个月时FLI均降低。全身脂肪和体重可预测两性的FLI。6M WLP后LEP和FLI的降低与sOB-r的变化无关。我们还证明，可通过总体脂肪量和体重（千克）对FLI进行无创预测。
