Bieber A J, Snow P M, Hortsch M, Patel N H, Jacobs J R, Traquina Z R, Schilling J, Goodman C S
Howard Hughes Medical Institute, University of California, Berkeley 94720.
Cell. 1989 Nov 3;59(3):447-60. doi: 10.1016/0092-8674(89)90029-9.
Drosophila neuroglian is an integral membrane glycoprotein that is expressed on a variety of cell types in the Drosophila embryo, including expression on a large subset of glial and neuronal cell bodies in the central and peripheral nervous systems and on the fasciculating axons that extend along them. Neuroglian cDNA clones were isolated by expression cloning. cDNA sequence analysis reveals that neuroglian is a member of the immunoglobulin superfamily. The extracellular portion of the protein consists of six immunoglobulin C2-type domains followed by five fibronectin type III domains. Neuroglian is closely related to the immunoglobulin-like vertebrate neural adhesion molecules and, among them, shows most extensive homology to mouse L1. Its homology to L1 and its embryonic localization suggest that neuroglian may play a role in neural and glial cell adhesion in the developing Drosophila embryo. We report here on the identification of a lethal mutation in the neuroglian gene.
果蝇神经粘连蛋白是一种整合膜糖蛋白，在果蝇胚胎的多种细胞类型中表达，包括中枢和外周神经系统中大部分神经胶质细胞和神经元细胞体以及沿其延伸的成束轴突上的表达。通过表达克隆分离出神经粘连蛋白cDNA克隆。cDNA序列分析表明，神经粘连蛋白是免疫球蛋白超家族的成员。该蛋白的细胞外部分由六个免疫球蛋白C2型结构域和五个纤连蛋白III型结构域组成。神经粘连蛋白与免疫球蛋白样脊椎动物神经粘附分子密切相关，其中与小鼠L1的同源性最为广泛。它与L1的同源性及其胚胎定位表明，神经粘连蛋白可能在发育中的果蝇胚胎的神经和神经胶质细胞粘附中发挥作用。我们在此报告神经粘连蛋白基因致死突变的鉴定。
