Department of Physiology, University of California, San Francisco, CA 94158.
Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158.
Proc Natl Acad Sci U S A. 2019 Mar 12;116(11):5126-5134. doi: 10.1073/pnas.1814456116. Epub 2019 Feb 25.
Sensory neurons perceive environmental cues and are important of organismal survival. Peripheral sensory neurons interact intimately with glial cells. While the function of axonal ensheathment by glia is well studied, less is known about the functional significance of glial interaction with the somatodendritic compartment of neurons. Herein, we show that three distinct glia cell types differentially wrap around the axonal and somatodendritic surface of the polymodal dendritic arborization (da) neuron of the peripheral nervous system for detection of thermal, mechanical, and light stimuli. We find that glial cell-specific loss of the chromatin modifier gene dATRX in the subperineurial glial layer leads to selective elimination of somatodendritic glial ensheathment, thus allowing us to investigate the function of such ensheathment. We find that somatodendritic glial ensheathment regulates the morphology of the dendritic arbor, as well as the activity of the sensory neuron, in response to sensory stimuli. Additionally, glial ensheathment of the neuronal soma influences dendritic regeneration after injury.
感觉神经元感知环境线索，对生物体的生存至关重要。外周感觉神经元与神经胶质细胞密切相互作用。虽然神经胶质细胞对轴突的鞘内作用的功能已经得到很好的研究，但对于神经胶质细胞与神经元的体树突区的相互作用的功能意义知之甚少。在此，我们展示了三种不同的神经胶质细胞类型在周围神经系统的多模态树突状分支（da）神经元的轴突和体树突表面上以不同的方式包裹，以检测热、机械和光刺激。我们发现，在神经鞘下胶质层中特异性缺失染色质修饰基因 dATRX 会导致体树突胶质鞘的选择性消除，从而使我们能够研究这种鞘的功能。我们发现，体树突胶质鞘调节树突分支的形态以及感觉神经元对感觉刺激的活性。此外，神经元胞体的神经胶质鞘包裹会影响损伤后的树突再生。
