Reyes-Rivera Jorge, Terrazas Teresa
Departamento de Botánica, Instituto de Biología, Universidad Nacional Autónoma de México, Mexico City, Mexico.
Instituto de Biología, Universidad Nacional Autónoma de México, Mexico City, Mexico.
Methods Mol Biol. 2017;1544:193-211. doi: 10.1007/978-1-4939-6722-3_14.
Fourier transform infrared spectroscopy (FTIR) is a simple non-destructive technique which allows the user to obtain quick and accurate information about the structure of the constituents of wood. High performance liquid chromatography (HPLC) is an analytical technique useful to determine the ratio of the lignin monomers obtained by the alkaline nitrobenzene oxidation method. Furthermore, lignin content has been commonly determined by wet chemical methods; Klason lignin determination is a quick and accessible method. Here, we detail the procedures for chemical analysis of the wood lignin using these techniques.
傅里叶变换红外光谱法（FTIR）是一种简单的无损技术，可让用户快速准确地获取有关木材成分结构的信息。高效液相色谱法（HPLC）是一种分析技术，有助于确定通过碱性硝基苯氧化法获得的木质素单体的比例。此外，木质素含量通常通过湿化学方法测定；克拉森木质素测定是一种快速且易于操作的方法。在此，我们详细介绍使用这些技术对木材木质素进行化学分析的程序。
