Decou Raphaël, Serk Henrik, Ménard Delphine, Pesquet Edouard
Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University, 901 87, Umeå, Sweden.
Department of Medical Biochemistry and Biophysics, Umeå University, 901 87, Umeå, Sweden.
Methods Mol Biol. 2017;1544:233-247. doi: 10.1007/978-1-4939-6722-3_17.
Lignin is a polyphenolic polymer specifically accumulating in the cell walls of xylem cells in higher vascular plants. Far from being homogeneous, the lignification of xylem cell walls varies in deposition site, quantity, composition and macromolecular conformation depending on the cell wall compartment, cell type, cell developmental stage and plant species. Here, we describe how confocal microspectroscopy methods using lignin autofluorescence can be used to evaluate the relative lignin amounts, its spatial distribution and composition at the cellular and sub-cellular levels in both isolated cells and histological cross-sections of plant tissues.
木质素是一种多酚聚合物，专门积累在高等维管植物木质部细胞的细胞壁中。木质部细胞壁的木质化远非均匀一致，其在沉积部位、数量、组成和大分子构象上存在差异，这取决于细胞壁区域、细胞类型、细胞发育阶段和植物物种。在这里，我们描述了如何使用基于木质素自发荧光的共聚焦显微光谱法，在植物组织的分离细胞和组织学横切面上，在细胞和亚细胞水平评估相对木质素含量、其空间分布和组成。
