Yu Zhaoyang, Xu Dongnian, Hu Jinbo, Chang Shanshan, Liu Gonggang, Huang Qiongtao, Han Jin, Li Ting, Liu Yuan, Wang Xiaodong Alice
College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.
Department of Research and Development Center, Yihua Lifestyle Technology Co., Ltd., Shantou 515834, China.
Polymers (Basel). 2023 Aug 1;15(15):3269. doi: 10.3390/polym15153269.
The autofluorescence phenomenon is an inherent characteristic of lignified cells. However, in the case of (), the autofluorescence is nearly imperceptible during occasional fluorescence observations. The aim of this study is to investigate the mechanism behind the quenching of lignin's autofluorescence in by conducting associated experiments. Notably, the autofluorescence image of observed using optical microscopy appears to be quite indistinct. Abundant extractives are found in the longitudinal parenchyma, fibers, and vessels of . Remarkably, when subjected to a benzene-alcohol extraction treatment, the autofluorescence of becomes progressively enhanced under a fluorescence microscope. Additionally, UV-Vis absorption spectra demonstrate that the extractives derived from exhibit strong light absorption within the wavelength range of 200-500 nm. This suggests that the abundant extractives in are probably responsible for the autofluorescence quenching observed in the cell walls. Moreover, the presence and quantity of these extractives have a significant impact on the fluorescence intensity of lignin in wood, resulting in a significant decrease therein. In future studies, it would be interesting to explore the role of complex compounds such as polyphenols or terpenoids, which are present in the abundant extractives, in interfering with the fluorescence quenching of lignin in .
自发荧光现象是木质化细胞的固有特征。然而，在（）的情况下，在偶尔的荧光观察期间自发荧光几乎难以察觉。本研究的目的是通过进行相关实验来探究（）中木质素自发荧光猝灭背后的机制。值得注意的是，使用光学显微镜观察到的（）的自发荧光图像似乎相当模糊。在（）的纵向薄壁组织、纤维和导管中发现了大量提取物。值得注意的是，当进行苯 - 醇萃取处理时，（）在荧光显微镜下的自发荧光逐渐增强。此外，紫外 - 可见吸收光谱表明，源自（）的提取物在200 - 500 nm波长范围内表现出强烈的光吸收。这表明（）中丰富的提取物可能是细胞壁中观察到的自发荧光猝灭的原因。此外，这些提取物的存在和数量对木材中木质素的荧光强度有显著影响，导致其显著降低。在未来的研究中，探索丰富提取物中存在的多酚或萜类等复杂化合物在干扰（）中木质素荧光猝灭方面的作用将是有趣的。
