Improving the Autofluorescence of Woody Cells via the Removal of Extractives.

The autofluorescence phenomenon is an inherent characteristic of lignified cells. However, in the case of (), the autofluorescence is nearly imperceptible during occasional fluorescence observations. The aim of this study is to investigate the mechanism behind the quenching of lignin's autofluorescence in by conducting associated experiments. Notably, the autofluorescence image of observed using optical microscopy appears to be quite indistinct. Abundant extractives are found in the longitudinal parenchyma, fibers, and vessels of . Remarkably, when subjected to a benzene-alcohol extraction treatment, the autofluorescence of becomes progressively enhanced under a fluorescence microscope. Additionally, UV-Vis absorption spectra demonstrate that the extractives derived from exhibit strong light absorption within the wavelength range of 200-500 nm. This suggests that the abundant extractives in are probably responsible for the autofluorescence quenching observed in the cell walls. Moreover, the presence and quantity of these extractives have a significant impact on the fluorescence intensity of lignin in wood, resulting in a significant decrease therein. In future studies, it would be interesting to explore the role of complex compounds such as polyphenols or terpenoids, which are present in the abundant extractives, in interfering with the fluorescence quenching of lignin in .