Giotto Biotech S.R.L. , Via Madonna del Piano 6, 50019 Sesto Fiorentino, Florence, Italy.
Department of Experimental and Clinical Medicine, University of Florence , Largo Brambilla 3, 50134 Florence, Italy.
Anal Chem. 2017 Jan 17;89(2):1054-1058. doi: 10.1021/acs.analchem.6b04318. Epub 2017 Jan 5.
In this letter, we propose an alternative, effective protocol for metabolomic characterization of biofluids based on their gelification and subsequent application of high-resolution magic angle spinning (HRMAS) H nuclear magnetic resonance (NMR). The sample handling is very rapid and reproducible, and much less than 40 μL of neat urine are needed to obtain a sample. Our results indicate that the HRMAS spectra of gelified urine encompass all metabolites in the NMR fingerprint, as observed by solution NMR. The proposed approach can be efficiently integrated into the NMR based metabolomics analyses routines: multivariate statistical analysis of both solution and HRMAS data produced very similar statistical models, with high classification accuracy. One of the key advantages offered by the gelification approach is the improved short-term (up to 24 h) preservation of nonfrozen HRMAS NMR gel urine samples compared to the solution samples, which could lead to an alternative way for transportation or domestic collection of biofluids, without the need of cold-storage and reducing the risks of leakage.
在这封信中，我们提出了一种替代的、有效的基于生物流体胶凝化及其随后应用高分辨率魔角旋转（HRMAS）H 核磁共振（NMR）的代谢组学特征分析方案。该样品处理方法非常快速且可重现，仅需不到 40 μL 的纯尿液即可获得样品。我们的结果表明，胶凝尿液的 HRMAS 光谱包含了溶液 NMR 观察到的 NMR 指纹图谱中的所有代谢物。所提出的方法可以有效地整合到基于 NMR 的代谢组学分析程序中：溶液和 HRMAS 数据的多变量统计分析产生了非常相似的统计模型，具有很高的分类准确性。胶凝方法的一个关键优势是，与溶液样品相比，非冷冻 HRMAS NMR 胶凝尿液样品在短期（长达 24 小时）内的保存得到了改善，这可能为生物流体的运输或国内采集提供了一种替代方法，无需冷藏，降低了泄漏的风险。
