Toujani Sonia, Mehiri Nadia, Hamzaoui Kamel, Hadded Hela, Ben Salah Nozha, Mjid Meriem, Ouahchi Yacine, Louzir Bechir, Daghfous Jalloul, Cherif Jouda, Beji Majed
Tunis Med. 2016 Jun;94(6):167-171.
Background - Many metalloproteinases (MMPs) play a role in the pathogenesis and modulation of the severity of asthma. MMP-9 is the predominant in asthma but other MMPs are involved such as the MMP-2. Aim - To determine the role of single nucleotide polymorphism of the gene MMP2 in susceptibility to asthma and its severity. Methods - Study case-control with prospectively enrolled patients with asthma and healthy subjects. We determined within two groups genotypes corresponding to the MMP2 polymorphism in -735C / T position, using a polymerase chain amplification technique associated with a polymorphism in the length of restriction fragments. Results - We included 150 patients with asthma and 150 healthy controls. Comparison of allele and genotype frequencies of the studied polymorphisms between patients and controls showed that there was no association between the SNP-735C / T and susceptibility to asthma and its severity. Conclusion - The role of MMP 2 in asthma remains unclear and no study has been conducted till date, to determine the role of MMP-2 -735C/T gene polymorphism in asthma. This study does not disprove such association. Further studies are needed to clarify the exact role the pathogenesis of asthma.
背景 - 许多金属蛋白酶（MMPs）在哮喘的发病机制及严重程度的调节中发挥作用。MMP - 9在哮喘中占主导地位，但其他MMPs如MMP - 2也参与其中。目的 - 确定MMP2基因单核苷酸多态性在哮喘易感性及其严重程度中的作用。方法 - 采用前瞻性纳入哮喘患者和健康受试者的病例对照研究。我们使用与限制性片段长度多态性相关的聚合酶链扩增技术，确定两组中对应于MMP2基因 - 735C / T位置多态性的基因型。结果 - 我们纳入了150例哮喘患者和150名健康对照。患者与对照之间所研究多态性的等位基因和基因型频率比较显示，SNP - 735C / T与哮喘易感性及其严重程度之间无关联。结论 - MMP 2在哮喘中的作用仍不明确，迄今为止尚未开展研究来确定MMP - 2 - 735C/T基因多态性在哮喘中的作用。本研究并未否定这种关联。需要进一步研究以阐明其在哮喘发病机制中的确切作用。
