Shibata N, Minouchi T, Hayashi Y, Shibata H, Ono T, Shimakawa H
Chem Pharm Bull (Tokyo). 1989 Jul;37(7):1877-80. doi: 10.1248/cpb.37.1877.
The effects of temperature, hematocrit (Hct), lipid level in plasma and cyclosporin A (CyA) level in whole blood on the concentration of CyA in plasma measured by high-performance liquid chromatography were studied in vitro. With rise in blood storage temperature before cells were removed, the concentration of CyA in plasma was increased in the temperature range between 10 degrees C and 37 degrees C, but was decreased between 4 degrees C and 10 degrees C. With rise in Hct, the concentration of CyA in plasma was decreased, and it was more markedly decreased at the blood storage temperature of 4 degrees C than at 37 degrees C. A lipid supplementation study showed that the concentration of CyA in plasma was increased with rise in plasma triglyceride level and in plasma cholesterol level at the storage temperature of 4 degrees C but not at 37 degrees C. Studies of the effect of CyA concentration in blood on the CyA distribution in blood demonstrated that the cellular/plasma concentration (C/P) ratio at low levels (less than 200 micrograms/ml) of plasma CyA ranged from 4 to 10 and was about 2 times higher than that at higher concentrations at 4 degrees C, but the ratio was relatively constant at 37 degrees C. The saturation capacity of the cellular fraction for CyA showed considerable individual variations, but there was no difference between the capacities at 4 degrees C and 37 degrees C. The separation of plasma after equilibration at 37 degrees C made it possible to avoid the variations in the distribution of CyA in whole blood associated with changes in Hct, lipid level in plasma and CyA level in whole blood, and to obtain a measurement reflecting the physiologically significant concentration of CyA in plasma.
体外研究了温度、血细胞比容（Hct）、血浆脂质水平和全血中环孢素A（CyA）水平对高效液相色谱法测定的血浆中CyA浓度的影响。在去除细胞前，随着血液储存温度升高，在10℃至37℃温度范围内血浆中CyA浓度升高，但在4℃至10℃之间降低。随着Hct升高，血浆中CyA浓度降低，且在4℃血液储存温度下比在37℃时降低更明显。脂质补充研究表明，在4℃储存温度下，随着血浆甘油三酯水平和血浆胆固醇水平升高，血浆中CyA浓度升高，但在37℃时未升高。血液中CyA浓度对血液中CyA分布影响的研究表明，在血浆CyA低水平（低于200微克/毫升）时，细胞/血浆浓度（C/P）比值在4至10之间，在4℃时比高浓度时约高2倍，但在37℃时该比值相对恒定。细胞组分对CyA的饱和容量显示出相当大的个体差异，但在4℃和37℃时的容量之间没有差异。在37℃平衡后分离血浆能够避免与Hct、血浆脂质水平和全血中CyA水平变化相关的全血中CyA分布变化，并获得反映血浆中CyA生理有效浓度的测量值。
