Felder R A, Mifflin T E, Bastani B
Clin Chem. 1986 Jul;32(7):1378-82.
We evaluated the use of the new iodinated ligand for the in vitro measurement of cyclosporin A by radioimmunoassay (RIA). Substitution of the iodinated cyclosporin (125I-CyA) for the corresponding tritium-labeled analog (3H-CyA) considerably simplifies and accelerates the currently available RIA, and improves its precision. Analysis of the respective dose-response curves showed that the 50% B0 value was lower for the 125I-CyA assay than for the 3H-CyA assay (37 vs 77 micrograms/L). Use of whole-blood specimens minimized interferences from temperature and hematocrit. We conclude that the use of 125I-CyA in a commercially available RIA for whole-blood specimens is accessible to most laboratories and provides rapid, reproducible data for management of transplant patients.
我们评估了新型碘化配体在通过放射免疫分析（RIA）体外测定环孢素A中的应用。用碘化环孢素（125I-CyA）替代相应的氚标记类似物（3H-CyA）可极大地简化并加速现有的RIA，并提高其精密度。对各自剂量反应曲线的分析表明，125I-CyA分析的50% B0值低于3H-CyA分析（37对77微克/升）。使用全血标本可将温度和血细胞比容的干扰降至最低。我们得出结论，在用于全血标本的市售RIA中使用125I-CyA，大多数实验室都可采用，并且可为移植患者的管理提供快速、可重复的数据。
