Protocol for Evaluating Neuronal Polarity in Murine Models.

The establishment of polarity is crucial for the physiology and wiring of neurons. Therefore, monitoring the axo-dendritic specification allows the mechanisms and signals associated with development, growth, and disease to be explored. Here, we describe major and minor steps to study polarity acquisition, using primary cultures of hippocampal neurons isolated from embryonic rat hippocampi, for monitoring. Furthermore, we use electroporated, GFP-expressing embryonic mouse brains for visualizing cortical neuron migration and polarization . Some underreported after-protocol steps are also included. For complete details on the use and execution of this protocol, please refer to Wilson et al. (2020).