Cambray-Deakin M A, Burgoyne R D
J Cell Biol. 1987 Jun;104(6):1569-74. doi: 10.1083/jcb.104.6.1569.
The distribution of acetylated alpha-tubulin in rat cerebellum was examined and compared with that of total alpha-tubulin and tyrosinated alpha-tubulin. From immunoperoxidase-stained vibratome sections of rat cerebellum it was found that acetylated alpha-tubulin, detectable with monoclonal 6-11B-1, was preferentially enriched in axons compared with dendrites. Parallel fiber axons, in particular, were labeled with 6-11B-1 yet unstained by an antibody recognizing tyrosinated alpha-tubulin, indicating that parallel fibers contain alpha-tubulin that is acetylated and detyrosinated. Axonal microtubules are known to be highly stable and the distribution of acetylated alpha-tubulin in other classes of stable microtubules suggests that acetylation and possibly detyrosination may play a role in the maintenance of stable populations of microtubules.
研究了大鼠小脑中乙酰化α-微管蛋白的分布，并将其与总α-微管蛋白和酪氨酸化α-微管蛋白的分布进行了比较。从大鼠小脑的免疫过氧化物酶染色振动切片中发现，用单克隆抗体6-11B-1可检测到的乙酰化α-微管蛋白，与树突相比，在轴突中优先富集。特别是平行纤维轴突，用6-11B-1标记，但不被识别酪氨酸化α-微管蛋白的抗体染色，这表明平行纤维含有乙酰化和去酪氨酸化的α-微管蛋白。已知轴突微管高度稳定，乙酰化α-微管蛋白在其他稳定微管类中的分布表明，乙酰化以及可能的去酪氨酸化可能在维持微管稳定群体中发挥作用。
