Shrivastava P, Kumar R, Yandigeri M S
Department of Environmental Microbiology, Babasaheb Bhimrao Ambedkar University (A Central Unversity), Lucknow 226025, Uttar Pradesh, India.
National Bureau of Agricultural Insect Resources, H.A. Farm Post, Bellary Road, Bengaluru 560024, India.
Saudi J Biol Sci. 2017 Jan;24(1):192-199. doi: 10.1016/j.sjbs.2015.12.004. Epub 2015 Dec 25.
A halotolerant actinobacterial strain isolated from salinity affected soil of Eastern Indo-Gangetic plains (IGP), Uttar Pradesh, India, was characterised for its antagonistic potential against by dual-culture assay. It was shown to effectively inhibit the growth of with an inhibition zone of 27 ± 1.33 mm. Further the actinobacterial strain was evaluated for its plant growth promoting (PGP) properties and its ability to produce biocontrol related extracellular enzymes amylase, protease, cellulase, chitinase, gelatinase and urease. The results revealed that the actinobacterial strain had PGP potential along with positive assay for amylase, chitinase and urease. The interaction study between antagonist strain and fungal pathogen, performed by scanning electron microscopy technique revealed that the actinobacterium was able to damage fungal mycelia may be due to chitinase, establishing its role as a potential antagonist against . The actinobacterial isolate was characterised by 16S rDNA gene sequencing, and was identified as genera. The identified gene sequence was deposited to NCBI GenBank with an accession number KP331758.
从印度北方邦东印度 - 恒河平原（IGP）受盐度影响的土壤中分离出的一株耐盐放线菌菌株，通过双培养试验对其对[具体病原菌未提及]的拮抗潜力进行了表征。结果表明它能有效抑制[具体病原菌未提及]的生长，抑菌圈为27 ± 1.33毫米。此外，还评估了该放线菌菌株的植物生长促进（PGP）特性及其产生与生物防治相关的胞外酶（淀粉酶、蛋白酶、纤维素酶、几丁质酶、明胶酶和脲酶）的能力。结果显示该放线菌菌株具有PGP潜力，且淀粉酶、几丁质酶和脲酶检测呈阳性。通过扫描电子显微镜技术对拮抗菌株与真菌病原体之间的相互作用进行研究，结果表明该放线菌可能由于几丁质酶而能够破坏真菌菌丝体，确立了其作为针对[具体病原菌未提及]的潜在拮抗剂的作用。通过16S rDNA基因测序对该放线菌分离株进行了表征，并鉴定为[具体属未提及]属。所鉴定的基因序列已存入NCBI基因库，登录号为KP331758。
