Liu Chao, Li Guo, Yang Nianting, Su Zhongwu, Zhang Shuiting, Deng Tengbo, Ren Shuling, Lu Shanhong, Tian Yongquan, Liu Yong, Qiu Yuanzheng
Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008 Hunan China.
Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008 Hunan China.
Cancer Cell Int. 2017 Jan 3;17:2. doi: 10.1186/s12935-016-0372-8. eCollection 2017.
Nasopharyngeal carcinoma (NPC) is a malignant epithelial carcinoma of the head and neck with strong ability of invasion and metastasis. Our previous study indicated that miR-324-3p, as a tumor-suppressive factor, could regulate radioresistance of NPC cells by targeting . The purpose of this study is to investigate the role of miR-324-3p on migration and invasion in NPC cells.
Quantitative real time PCR was applied to measure the expression level of miR-324-3p and mRNA in both cells and tissues, and the expression level of protein was determined by western blotting. The capacity of migration and invasion were tested by using wound healing and transwell invasion assay.
Ectopic expression of miR-324-3p or silencing its target gene could dramatically suppress migration and invasion capacity of NPC cells. Meanwhile, the alterations of miR-324-3p in NPC cells could influence the expression level of the biomarkers of epithelial-mesenchymal transition (EMT), including E-cadherin and Vimentin. Moreover, the expression of miR-324-3p was obviously downregulated and was significantly upregulated in NPC tissues. The expression levels of miR-324-3p and were closely correlated with T stage, clinic stage and cervical lymph node metastasis of NPC ( < 0.05).
miR-324-3p could suppress the migration and invasion of NPC by targeting and the miR-324-3p/WNT2B pathway possibly provide new potential therapeutic clues for NPC.
鼻咽癌（NPC）是一种头颈部恶性上皮癌，具有很强的侵袭和转移能力。我们之前的研究表明，miR-324-3p作为一种肿瘤抑制因子，可通过靶向……调节NPC细胞的放射抗性。本研究旨在探讨miR-324-3p在NPC细胞迁移和侵袭中的作用。
应用定量实时PCR检测细胞和组织中miR-324-3p和……mRNA的表达水平，通过蛋白质印迹法测定……蛋白的表达水平。采用伤口愈合实验和Transwell侵袭实验检测迁移和侵袭能力。
miR-324-3p的异位表达或沉默其靶基因……可显著抑制NPC细胞的迁移和侵袭能力。同时，NPC细胞中miR-324-3p的改变可影响上皮-间质转化（EMT）生物标志物的表达水平，包括E-钙黏蛋白和波形蛋白。此外，NPC组织中miR-324-3p的表达明显下调，……显著上调。miR-324-3p和……的表达水平与NPC的T分期、临床分期及颈部淋巴结转移密切相关（P<0.05）。
miR-324-3p可通过靶向……抑制NPC的迁移和侵袭，miR-324-3p/WNT2B通路可能为NPC提供新的潜在治疗线索。
