Liu Chao, Li Guo, Ren Shuling, Su Zhongwu, Wang Yunyun, Tian Yongquan, Liu Yong, Qiu Yuanzheng
Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital Central South University, Changsha, Hunan 410008, P.R. China.
Otolaryngology Major Disease Research Key Laboratory of Hunan, Xiangya Hospital Central South University, Changsha, Hunan 410008, P.R. China.
Oncol Lett. 2017 Apr;13(4):2631-2636. doi: 10.3892/ol.2017.5778. Epub 2017 Feb 24.
MicroRNAs (miRs) have been recognised as important regulators of malignant behaviour in different types of human cancer, including nasopharyngeal carcinoma (NPC). A previous study by our group revealed that miR-185-3p regulates the radioresistance of NPC cells. The present study aimed to investigate the effect of miR-185-3p on NPC invasion and metastasis. Human NPC CNE-2 and 5-8F cell lines were transfected with a miR-185-3p mimic and miR-185-3p inhibitor, respectively, and their effects on the invasion and metastasis of these cells was assessed using a wound healing assay and Matrigel invasion assay. The target gene of miR-185-3p, Wnt family member 2B ( was silenced in 5-8F cells using siRNA in order to investigate its function in NPC. Data from the present study demonstrated that the expression of miR-185-3p was the highest in 5-8F and lowest in CNE-2 cells out of a range of NPC cell lines. Following the transfection of miR-185-3p mimic into CNE-2 cells, the wound healing and Matrigel invasion assays indicated that the migration and invasion ability of CNE-2 cells was significantly reduced compared with the negative control group. In addition, the inhibition of miR-185-3p in 5-8F cells significantly increased the capacity for migration and invasion. Furthermore, silencing expression resulted in a significant reduction in the invasion and metastasis in 5-8F cells. The inhibition of miR-185-3p, which promotes invasion and metastasis, could be reversed through the silencing of in 5-8F cells. The results of the present study indicate that miR-185-3p mediates the invasion and metastasis of NPC by targeting .
微小RNA（miR）已被公认为是包括鼻咽癌（NPC）在内的不同类型人类癌症中恶性行为的重要调节因子。我们小组之前的一项研究表明，miR-185-3p可调节NPC细胞的放射抗性。本研究旨在探讨miR-185-3p对NPC侵袭和转移的影响。分别用miR-185-3p模拟物和miR-185-3p抑制剂转染人NPC CNE-2和5-8F细胞系，并使用伤口愈合试验和基质胶侵袭试验评估它们对这些细胞侵袭和转移的影响。为了研究miR-185-3p的靶基因Wnt家族成员2B（ ）在NPC中的功能，使用小干扰RNA（siRNA）在5-8F细胞中使其沉默。本研究数据表明，在一系列NPC细胞系中，miR-185-3p的表达在5-8F细胞中最高，在CNE-2细胞中最低。将miR-185-3p模拟物转染到CNE-2细胞后，伤口愈合和基质胶侵袭试验表明，与阴性对照组相比，CNE-2细胞的迁移和侵袭能力显著降低。此外，在5-8F细胞中抑制miR-185-3p可显著提高迁移和侵袭能力。此外，沉默 表达导致5-8F细胞的侵袭和转移显著减少。在5-8F细胞中通过沉默 可逆转促进侵袭和转移的miR-185-3p的抑制作用。本研究结果表明，miR-185-3p通过靶向 介导NPC的侵袭和转移。
