Zhang Rui, Piao Mei Jing, Oh Min Chang, Park Jeong Eon, Shilnikova Kristina, Moon Yu Jin, Kim Dong Hyun, Jung Uhee, Kim In Gyu, Hyun Jin Won
Department of Biochemistry, Jeju National University School of Medicine, Jeju, Korea; School of Medical Science and Laboratory Medicine, Jiangsu University, Jiangsu, China.
Department of Biochemistry, Jeju National University School of Medicine, Jeju, Korea.
J Cancer Prev. 2016 Dec;21(4):257-263. doi: 10.15430/JCP.2016.21.4.257. Epub 2016 Dec 30.
Isoflavones are biologically active compounds that occur naturally in a variety of plants, with relatively high levels in soybean. Tectorigenin, an isoflavone, protects against hydrogen peroxide (HO)-induced cell damage. However, the underlying mechanism is unknown.
The MTT assay was performed to determine cell viability. Catalase activity was assessed by determining the amount of enzyme required to degrade 1 μM HO. Protein expression of catalase, phospho-extracellular signal-regulated kinase (ERK), IκB-α, and NF-κB were evaluated by Western blot analysis. A mobility shift assay was performed to assess the DNA-binding ability of NF-κB. Transient transfection and a NF-κB luciferase assay were performed to assess transcriptional activity.
Tectorigenin reduced HO-induced death of Chinese hamster lung fibroblasts (V79-4). In addition, tectorigenin increased the activity and protein expression of catalase. Blockade of catalase activity attenuated the protective effect of tectorigenin against oxidative stress. Furthermore, tectorigenin enhanced phosphorylation of ERK and nuclear expression of NF-κB, while inhibition of ERK and NF-κB attenuated the protective effect of tectorigenin against oxidative stress.
Tectorigenin protects cells against oxidative damage by activating catalase and modulating the ERK and NF-κB signaling pathway.
异黄酮是一类生物活性化合物，天然存在于多种植物中，大豆中含量相对较高。鸢尾黄酮作为一种异黄酮，可保护细胞免受过氧化氢（H₂O₂）诱导的损伤。然而，其潜在机制尚不清楚。
采用MTT法测定细胞活力。通过测定降解1 μM H₂O₂所需的酶量来评估过氧化氢酶活性。采用蛋白质免疫印迹法检测过氧化氢酶、磷酸化细胞外信号调节激酶（ERK）、IκB-α和核因子κB（NF-κB）的蛋白表达。采用凝胶迁移试验评估NF-κB的DNA结合能力。通过瞬时转染和NF-κB荧光素酶试验评估转录活性。
鸢尾黄酮减少了H₂O₂诱导的中国仓鼠肺成纤维细胞（V79-4）死亡。此外，鸢尾黄酮增加了过氧化氢酶的活性和蛋白表达。阻断过氧化氢酶活性减弱了鸢尾黄酮对氧化应激的保护作用。此外，鸢尾黄酮增强了ERK的磷酸化和NF-κB的核表达，而抑制ERK和NF-κB减弱了鸢尾黄酮对氧化应激的保护作用。
鸢尾黄酮通过激活过氧化氢酶并调节ERK和NF-κB信号通路来保护细胞免受氧化损伤。
