Department of Chemistry and Research Institute of Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea.
Food Safety Center, Lotte Confectionery Co., Ltd., Seoul 07207, Republic of Korea.
Sci Rep. 2017 Jan 5;7:40305. doi: 10.1038/srep40305.
Overuse of antibiotics has caused serious problems, such as appearance of super bacteria, whose accumulation in the human body through the food chain is a concern. Kanamycin is a common antibiotic used to treat diverse infections; however, residual kanamycin can cause many side effects in humans. Thus, development of an ultra-sensitive, precise, and simple detection system for residual kanamycin in food products is urgently needed for food safety. In this study, we identified kanamycin-binding aptamers via a new screening method, and truncated variants were analyzed for optimization of the minimal sequence required for target binding. We found various aptamers with high binding affinity from 34.7 to 669 nanomolar K values with good specificity against kanamycin. Furthermore, we developed a reduced graphene oxide (RGO)-based fluorescent aptasensor for kanamycin detection. In this system, kanamycin was detected at a concentration as low as 1 pM (582.6 fg/mL). In addition, this method could detect kanamycin accurately in kanamycin-spiked blood serum and milk samples. Consequently, this simple, rapid, and sensitive kanamycin detection system with newly structural and functional analysis aptamer exhibits outstanding detection compared to previous methods and provides a new possibility for point of care testing and food safety.
抗生素的过度使用已经导致了严重的问题，例如超级细菌的出现，这些超级细菌通过食物链在人体内的积累令人担忧。卡那霉素是一种常用于治疗各种感染的常用抗生素；然而，残留的卡那霉素会给人类带来许多副作用。因此，迫切需要开发一种超灵敏、精确和简单的检测系统，以检测食品中残留的卡那霉素，确保食品安全。在这项研究中，我们通过一种新的筛选方法鉴定了卡那霉素结合适体，并分析了截短变体，以优化靶标结合所需的最小序列。我们从 34.7 到 669 纳摩尔 K 值范围内发现了各种具有高结合亲和力的适体，对卡那霉素具有良好的特异性。此外，我们还开发了一种基于还原氧化石墨烯 (RGO) 的荧光适体传感器来检测卡那霉素。在这个系统中，卡那霉素的检测浓度低至 1 pM（582.6 fg/mL）。此外，该方法可以准确检测卡那霉素掺杂的血清和牛奶样品中的卡那霉素。因此，与以前的方法相比，这种具有新结构和功能分析适体的简单、快速和灵敏的卡那霉素检测系统具有出色的检测性能，为即时检测和食品安全提供了新的可能性。
