Shandong Provincial Key Laboratory of Chemical Sensing & Analysis, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
School of Environmental science and Engineering, Qilu University of Technology, Jinan 250353, China.
Biosens Bioelectron. 2016 Mar 15;77:752-8. doi: 10.1016/j.bios.2015.10.050. Epub 2015 Oct 23.
An ultrasensitive electrochemical aptasensor for the quantitative detection of kanamycin antibiotic was fabricated based on a novel signal amplification strategy. This aptasensor was developed using thionine functionalized graphene (GR-TH) and hierarchical nanoporous (HNP) PtCu alloy as biosensing substrates for the first time. HNP-PtCu alloy with controllable bimodal ligament/pore distributions was successfully prepared by two-step dealloying of a well-designed PtCuAl precursor alloy combined with an annealing operation. GR-TH composite was synthesized by one-step reduction of graphene oxide (GO) in TH solution. Greatly amplified sensitivity was achieved by using GR-TH/HNP-PtCu composite owing to its large specific surface and good electron-transfer ability. Under the optimized conditions, the proposed aptasensor exhibited a high sensitivity and a wider linearity to kanamycin in the range 5 × 10(-7)-5 × 10(-2) μgmL(-1) with a low detection limit of 0.42 pgmL(-1). This aptasensor also displayed a satisfying electrochemical performance with good stability, selectivity and reproducibility. The as-prepared aptasensor was successfully used for the determination of kanamycin in animal derived food.
基于一种新颖的信号放大策略，制备了用于定量检测卡那霉素抗生素的超灵敏电化学适体传感器。该适体传感器是首次使用噻吩功能化石墨烯（GR-TH）和分级纳米多孔（HNP）PtCu 合金作为生物传感基底开发的。通过两步脱合金法（使用设计良好的 PtCuAl 前驱体合金结合退火操作）成功制备了具有可控双模态连接/孔分布的 HNP-PtCu 合金。通过在 TH 溶液中一步还原氧化石墨烯（GO）合成了 GR-TH 复合材料。由于其大的比表面积和良好的电子转移能力，使用 GR-TH/HNP-PtCu 复合材料实现了灵敏度的极大提高。在优化条件下，所提出的适体传感器对卡那霉素在 5×10(-7)-5×10(-2)μgmL(-1)范围内表现出高灵敏度和更宽的线性范围，检测限低至 0.42pgmL(-1)。该适体传感器还表现出良好的电化学性能，具有良好的稳定性、选择性和重现性。所制备的适体传感器成功用于动物源性食品中卡那霉素的测定。
