Fedotova Julia, Pivina Svetlana, Sushko Anastasia
Laboratory of Neuroendocrinology, I.P. Pavlov Institute of Physiology of the Russian Academy of Sciences, 6 Emb. Makarova, Saint Petersburg 199034, Russia.
Laboratory of Comparative Somnology and Neuroendocrinology, I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences, 44 Tores pr., Saint Petersburg 194223, Russia.
Nutrients. 2017 Jan 3;9(1):28. doi: 10.3390/nu9010028.
The present preclinical study was created to determine the therapeutic effects of vitamin D hormone treatment as an adjunctive therapy alone or in a combination with low dose of 17β-estradiol (17β-E₂) on anxiety-like behavior in female rats with long-term absence of estrogen. Accordingly, the aim of the current study was to examine the effects of chronic cholecalciferol administration (1.0, 2.5 or 5.0 mg/kg subcutaneously, SC, once daily, for 14 days) on the anxiety-like state after long-term ovariectomy in female rats. Twelve weeks postovariectomy, cholecalciferol was administered to ovariectomized (OVX) rats and OVX rats treated with 17β-E₂ (0.5 µg/rat SC, once daily, for 14 days). Anxiety-like behavior was assessed in the elevated plus maze (EPM) and the light/dark test (LDT), and locomotor and grooming activities were tested in the open field test (OFT). Cholecalciferol at two doses of 1.0 and 2.5 mg/kg alone or in combination with 17β-E₂ produced anxiolytic-like effects in OVX rats as evidenced in the EPM and the LDT, as well as increased grooming activity in the OFT. Our results indicate that cholecalciferol, at two doses of 1.0 and 2.5 mg/kg, has a profound anxiolytic-like effects in the experimental rat model of long-term estrogen deficiency.
本临床前研究旨在确定维生素D激素单独作为辅助治疗或与低剂量17β-雌二醇（17β-E₂）联合使用对长期缺乏雌激素的雌性大鼠焦虑样行为的治疗效果。因此，本研究的目的是检验慢性给予胆钙化醇（皮下注射1.0、2.5或5.0 mg/kg，每日一次，共14天）对雌性大鼠长期卵巢切除术后焦虑样状态的影响。卵巢切除术后12周，对去卵巢（OVX）大鼠和用17β-E₂治疗的OVX大鼠（皮下注射0.5 µg/只，每日一次，共14天）给予胆钙化醇。在高架十字迷宫（EPM）和明暗试验（LDT）中评估焦虑样行为，并在旷场试验（OFT）中测试运动和梳理活动。1.0和2.5 mg/kg两种剂量的胆钙化醇单独使用或与17β-E₂联合使用，在EPM和LDT中均显示出对OVX大鼠有抗焦虑样作用，并且在OFT中增加了梳理活动。我们的结果表明，1.0和2.5 mg/kg两种剂量的胆钙化醇在长期雌激素缺乏的实验大鼠模型中具有显著的抗焦虑样作用。
