Fedotova Julia
Laboratory of Neuroendocrinology, I. P. Pavlov Institute of Physiology of the Russian Academy of Science, 6 Emb. Makarova, Saint Petersburg 199034, Russia.
Biomed Res Int. 2014;2014:847821. doi: 10.1155/2014/847821. Epub 2014 Feb 23.
The aim of this study was to explore effects on anxiety-like behavior of D1 dopamine receptor agonist, SKF-38393, and of D1 dopamine receptor antagonist, SCH-23390, given alone or in combination with a low dose of 17β-estradiol (17β-E2) to ovariectomized (OVX) rats. Two weeks after surgery, OVX rats began 14 days of treatment with the vehicle, a low dose of 17 β -E2 (5.0 μg/rat, s.c.), SKF-38393 (0.1 mg/kg, i.p.), SCH-23390 (0.1 mg/kg, i.p.), SKF-38393 plus 17β-E2, or SCH-23390 plus 17β-E2. The animals were tested in the black and white model (BWM) and the open field test (OFT). SCH-23390 (0.1 mg/kg, i.p.) alone or in a combination with a low dose of 17β-E2 (5.0 μg/rat, s.c.) resulted in anxiolytic-like effect in OVX rats in the BWM. Repeated treatment with SCH-23390 and 17β-E2 profoundly increased anxiolytic-like effect of single substances exerted per se. Coadministration of SCH-23390 with 17β-E2 increased frequency of rearing and grooming in OVX rats in OFT. SKF-38393 (0.1 mg/kg, i.p.) treatment failed to alter anxiety-like behavior in OVX rats in the BWM. The results of the present study suggest that 17β-E2 and SCH-23390 interact to exert anxiolytic-like action and that each of these drugs can potentiate effects of each other.
本研究的目的是探究单独给予或与低剂量17β-雌二醇（17β-E2）联合给予D1多巴胺受体激动剂SKF-38393和D1多巴胺受体拮抗剂SCH-23390对去卵巢（OVX）大鼠焦虑样行为的影响。手术两周后，OVX大鼠开始接受为期14天的如下处理：溶剂、低剂量17β-E2（5.0μg/大鼠，皮下注射）、SKF-38393（0.1mg/kg，腹腔注射）、SCH-23390（0.1mg/kg，腹腔注射）、SKF-38393加17β-E2或SCH-23390加17β-E2。对动物进行黑白箱模型（BWM）和旷场试验（OFT）测试。单独给予SCH-23390（0.1mg/kg，腹腔注射）或与低剂量17β-E2（5.0μg/大鼠，皮下注射）联合给予时，可使OVX大鼠在BWM中产生抗焦虑样效应。重复给予SCH-23390和17β-E2可显著增强单一物质本身所产生抗焦虑样效应。在OFT中，将SCH-23390与17β-E2联合给药可增加OVX大鼠的竖毛和理毛频率。给予SKF-38393（0.1mg/kg，腹腔注射）未能改变OVX大鼠在BWM中的焦虑样行为。本研究结果表明，17β-E2和SCH-23390相互作用发挥抗焦虑样作用，且这两种药物均可增强彼此的效应。
