McDonnell Genome Institute, Washington University, St. Louis, Missouri, USA.
Nat Protoc. 2017 Feb;12(2):213-218. doi: 10.1038/nprot.2016.182. Epub 2017 Jan 5.
Recent advances in the field of genomics have largely been due to the ability to sequence DNA at increasing throughput and decreasing cost. DNA sequencing was first introduced in 1977, and next-generation sequencing technologies have been available only during the past decade, but the diverse experiments and corresponding analyses facilitated by these techniques have transformed biological and biomedical research. Here, I review developments in DNA sequencing technologies over the past 10 years and look to the future for further applications.
近年来，基因组学领域的进展在很大程度上要归功于能够以越来越高的通量和越来越低的成本对 DNA 进行测序。DNA 测序于 1977 年首次推出，而新一代测序技术仅在过去十年中才得以应用，但这些技术所带来的多样化实验和相应分析已经改变了生物和生物医学研究。在这里，我回顾了过去 10 年中 DNA 测序技术的发展，并展望了未来的进一步应用。
