1 Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences Brno , Brno, Czech Republic .
2 Biology Centre, Institute of Parasitology , Czech Academy of Sciences, České Budějovice, Czech Republic .
Vector Borne Zoonotic Dis. 2017 Jan;17(1):66-72. doi: 10.1089/vbz.2016.1963.
Vector-borne protists of the genus Hepatozoon belong to the apicomplexan suborder Adeleorina. The taxonomy of Hepatozoon is unsettled and different phylogenetic clades probably represent evolutionary units deserving the status of separate genera. Throughout our review, we focus on the monophyletic assemblage of Hepatozoon spp. from carnivores, classified as Hepatozoon sensu stricto that includes important pathogens of domestic and free-ranging canine and feline hosts. We provide an overview of diagnostic methods and approaches from classical detection in biological materials, through serological tests to nucleic acid amplification tests (NAATs). Critical review of used primers for the 18S rDNA is provided, together with information on individual primer pairs. Extension of used NAATs target to cover also mitochondrial genes is suggested as a key step in understanding the diversity and molecular epidemiology of Hepatozoon infections in mammals.
属噬肝原生动物的虫媒原生动物属于顶复亚目 Adeleorina。噬肝原生动物的分类尚未确定，不同的系统发育分支可能代表具有单独属地位的进化单位。在我们的综述中，我们专注于从肉食动物中分离出的噬肝原生动物属的单系集合，被归类为噬肝原生动物属，其中包括对家养和自由放养犬和猫宿主的重要病原体。我们提供了从生物材料中的经典检测到血清学检测再到核酸扩增检测（NAAT）的诊断方法和方法的概述。我们还提供了对用于 18S rDNA 的引物的批判性审查，以及有关单个引物对的信息。建议将用于 NAAT 的目标扩展到覆盖线粒体基因，作为理解哺乳动物中噬肝原生动物感染的多样性和分子流行病学的关键步骤。
