评估大鼠眼部功能血流成像的麻醉方案。
Evaluating anesthetic protocols for functional blood flow imaging in the rat eye.
机构信息
Massachusetts Institute of Technology, Research Laboratory of Electronics, Department of Electrical Engineering and Computer Science, 50 Vassar Street, Cambridge 02139, United States.
Harvard Medical School, Massachusetts General Hospital, Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, 13th Street, Charlestown 02129, United States.
出版信息
J Biomed Opt. 2017 Jan 1;22(1):16005. doi: 10.1117/1.JBO.22.1.016005.
The purpose of this study is to evaluate the suitability of five different anesthetic protocols (isoflurane, isoflurane–xylazine, pentobarbital, ketamine–xylazine, and ketamine–xylazine–vecuronium) for functional blood flow imaging in the rat eye. Total retinal blood flow was measured at a series of time points using an ultrahigh-speed Doppler OCT system. Additionally, each anesthetic protocol was qualitatively evaluated according to the following criteria: (1) time-stability of blood flow, (2) overall rate of blood flow, (3) ocular immobilization, and (4) simplicity. We observed that different anesthetic protocols produced markedly different blood flows. Different anesthetic protocols also varied with respect to the four evaluated criteria. These findings suggest that the choice of anesthetic protocol should be carefully considered when designing and interpreting functional blood flow studies in the rat eye.
本研究旨在评估五种不同麻醉方案（异氟烷、异氟烷-二甲苯胺噻嗪、戊巴比妥、氯胺酮-二甲苯胺噻嗪和氯胺酮-二甲苯胺噻嗪-维库溴铵）在大鼠眼部功能血流成像中的适用性。使用超高速多普勒 OCT 系统在一系列时间点测量总视网膜血流。此外，根据以下标准对每种麻醉方案进行定性评估：（1）血流的时间稳定性，（2）整体血流率，（3）眼球固定，（4）简单性。我们观察到不同的麻醉方案产生了明显不同的血流。不同的麻醉方案在四个评估标准上也有所不同。这些发现表明，在设计和解释大鼠眼部功能血流研究时，应仔细考虑麻醉方案的选择。
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