Kawamura Jumpei, Kamoshida Shingo, Shimakata Takaaki, Hayashi Yurie, Sakamaki Kuniko, Denda Tamami, Kawai Kenji, Kuwao Sadahito
Department of Diagnostic Pathology and Cytology, Higashiyamato Hospital, Higashiyamato, Tokyo, Japan.
Laboratory of Pathology, Department of Medical Biophysics, Kobe University Graduate School of Health Sciences, Kobe, Hyogo, Japan.
Cancer Cytopathol. 2017 Apr;125(4):277-282. doi: 10.1002/cncy.21816. Epub 2017 Jan 5.
Intraoperative diagnosis of central nervous system (CNS) tumors provides critical guidance to surgeons in the determination of surgical resection margins and treatment. The techniques and preparations used for the intraoperative diagnosis of CNS tumors include frozen sectioning and cytologic methods (squash smear and touch imprint). Cytologic specimens, which do not have freezing artifacts, are important as an adjuvant tool to frozen sections. However, if the amount of submitted tissue samples is limited, then it is difficult to prepare both frozen sections and squash smears or touch imprint specimens from a single sample at the same time. Therefore, the objective of this study was to derive cells directly from filter paper on which tumor samples are placed.
The authors established the filter paper-assisted cell transfer (FaCT) smear technique, in which tumor cells are transferred onto a glass slide directly from the filter paper sample spot after the biopsy is removed.
Cell yields and diagnostic accuracy of the FaCT smears were assessed in 40 CNS tumors. FaCT smears had ample cell numbers and well preserved cell morphology sufficient for cytologic diagnosis, even if the submitted tissues were minimal. The overall diagnostic concordance rates between frozen sections and FaCT smears were 90% and 87.5%, respectively (no significant differences). When combining FaCT smears with frozen sections, the diagnostic concordance rate rose to 92.5%.
The current results suggest that the FaCT smear technique is a simple and effective processing method that has significant value for intraoperative diagnosis of CNS tumors. Cancer Cytopathol 2017;125:277-282. © 2016 American Cancer Society.
中枢神经系统（CNS）肿瘤的术中诊断为外科医生确定手术切除边缘和治疗提供关键指导。用于CNS肿瘤术中诊断的技术和准备工作包括冷冻切片和细胞学方法（压片涂片和触摸印片）。没有冷冻假象的细胞学标本作为冷冻切片的辅助工具很重要。然而，如果提交的组织样本量有限，那么很难同时从单个样本制备冷冻切片和压片涂片或触摸印片标本。因此，本研究的目的是直接从放置肿瘤样本的滤纸上获取细胞。
作者建立了滤纸辅助细胞转移（FaCT）涂片技术，即在活检组织取出后，将肿瘤细胞直接从滤纸样本点转移到载玻片上。
在40例CNS肿瘤中评估了FaCT涂片的细胞产量和诊断准确性。即使提交的组织很少，FaCT涂片也有足够的细胞数量和保存良好的细胞形态，足以进行细胞学诊断。冷冻切片和FaCT涂片之间的总体诊断符合率分别为90%和87.5%（无显著差异）。当将FaCT涂片与冷冻切片结合使用时，诊断符合率升至92.5%。
目前的结果表明，FaCT涂片技术是一种简单有效的处理方法，对CNS肿瘤的术中诊断具有重要价值。《癌症细胞病理学》2017年；125:277 - 282。©2016美国癌症协会。
