Bao Qian, Nishimura Norihito, Kamata Hirotoshi, Furue Kentaro, Ono Yuki, Hosomi Masaaki, Terada Akihiko
Department of Chemical Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka, Koganei, Tokyo 184-8588, Japan.
Functional Materials Group, Showa Denko K.K., 5-1 Okawa, Kawasaki, Kanagawa 210-0858, Japan.
Colloids Surf B Biointerfaces. 2017 Mar 1;151:363-371. doi: 10.1016/j.colsurfb.2016.12.020. Epub 2016 Dec 15.
Fluorinated polymers generally function as antibacterial agents, but their anti-biofilm effect remains unresolved. This study investigates the efficacy of fluoropolymers containing 2,3,5,6-tetrafluoro-p-phenylenedimethanol (TFPDM) in preventing biofilm formation by Bacillus subtilis and Escherichia coli (Gram-positive and Gram-negative bacterial species). To this end, TFPDM-based acrylate and epoxy polymers (AF and EF, respectively) and their structural analogues without TFPDM (A and E, respectively) were synthesized. All polymers were coated onto polyethylene terephthalate (PET) sheets. Relative to pristine PET, sheets coated with AF reduced the initial bacterial adhesion (72h) and biofilm formation (30days) of B. subtilis by 27.6% and 68.7% and of E. coli by 89.2% and 93.8%, respectively. The comparable antibacterial and anti-biofilm efficacies were obtained by sheets with EF. The biofilm detachment was substantially facilitated from the AF, compared with the structural analogue without TFPDM (A). In this comprehensive study, the bacterial adhesion and subsequent biofilm formation were prevented by TFPDM-containing polymers effectively.
含氟聚合物通常起抗菌剂的作用，但其抗生物膜效果仍未明确。本研究调查了含有2,3,5,6 - 四氟对苯二甲醇（TFPDM）的含氟聚合物对枯草芽孢杆菌和大肠杆菌（革兰氏阳性和革兰氏阴性细菌种类）生物膜形成的预防效果。为此，合成了基于TFPDM的丙烯酸酯和环氧树脂聚合物（分别为AF和EF）及其不含TFPDM的结构类似物（分别为A和E）。所有聚合物均涂覆在聚对苯二甲酸乙二酯（PET）片材上。相对于原始PET，涂有AF的片材使枯草芽孢杆菌的初始细菌粘附（72小时）和生物膜形成（30天）分别降低了27.6%和68.7%，使大肠杆菌的分别降低了89.2%和93.8%。涂有EF的片材也获得了类似的抗菌和抗生物膜效果。与不含TFPDM的结构类似物（A）相比，AF能显著促进生物膜的脱离。在这项全面研究中，可以有效防止含TFPDM的聚合物与细菌粘附及随后的生物膜形成。
