Aggarwal S J, Shah S J, Diller K R, Baxter C R
Department of Mechanical Engineering, University of Texas, Austin 78712-1084.
Comput Biol Med. 1989;19(4):245-61. doi: 10.1016/0010-4825(89)90012-7.
Computer vision techniques implemented on an IBM PC/AT have been applied to the study of microvascular permeability and interstitial diffusion in dorsal skin flap chamber preparations of hamsters. Experimental data was obtained for the leakage of fluorescent labelled dextran (70,000 daltons) after a precisely controlled mild degree of localized thermal trauma and compared with control data acquired prior to burn injury. Computer vision analysis techniques were applied to convert the fluorescent images into two-dimensional concentration maps. Interstitial diffusion coefficient values were computed from measured extravascular concentration profiles around a vessel of interest, assuming cylindrical or rectangular geometry, and optimally fitting a diffusion model to the data. An increase in the apparent diffusivity after mild thermal trauma was observed. Novel techniques were applied to solve hardware problems related to data acquisition and analysis, and a new library of software was developed to handle specific image processing requirements.
在IBM PC/AT上实现的计算机视觉技术已应用于仓鼠背部皮瓣腔室制剂中微血管通透性和间质扩散的研究。在精确控制的轻度局部热创伤后，获得了荧光标记葡聚糖（70,000道尔顿）渗漏的实验数据，并与烧伤损伤前获取的对照数据进行了比较。应用计算机视觉分析技术将荧光图像转换为二维浓度图。假设为圆柱形或矩形几何形状，并对数据进行扩散模型的最优拟合，从感兴趣血管周围测量的血管外浓度分布计算间质扩散系数值。观察到轻度热创伤后表观扩散率增加。应用新技术解决与数据采集和分析相关的硬件问题，并开发了一个新的软件库来处理特定的图像处理要求。
