Sánchez-Villavicencio Mayra L, Vinqvist-Tymchuk Melinda, Kalt Wilhelmina, Matar Chantal, Alarcón Aguilar Francisco J, Escobar Villanueva Maria Del Carmen, Haddad Pierre S
Natural Health Products and Metabolic Diseases Laboratory and CIHR Team in Aboriginal Anti-diabetic Medicines, Department of Pharmacology and Physiology, Faculty of Medicine, Université de Montréal, C.P. 6128 Succ. Centre-ville / P.O. Box 6128, Downtown Postal Station, Montreal, H3C 3 J7, QC, Canada.
Postgrade Program in Experimental Biology, Division of Health and Biological Sciences, Metropolitan Autonomous University of Iztapalapa, Mexico DF, Mexico.
BMC Complement Altern Med. 2017 Jan 6;17(1):24. doi: 10.1186/s12906-016-1519-9.
Obesity and Type 2 diabetes have reached epidemic status worldwide. Wild lowbush blueberry (Vaccinium angustifolium Aiton) is a plant of the North American Aboriginal traditional pharmacopeia with antidiabetic potential, especially when it is fermented with Serratia vaccinii.
A phytochemical fractionation scheme was used to identify potential bioactive compounds as confirmed by HPLC retention times and UV-Vis spectra. 3 T3-L1 cells were differentiated for 7 days with either Normal Blueberry Extract (NBE), Fermented Blueberry Extract (FBE/F1), seven fractions and four pure compounds. Triglyceride content was measured. Examination of selected intracellular signalling components (p-Akt, p-AMPK) and transcriptional factors (SREBP-1c and PPARγ) was carried out by Western blot analysis.
The inhibitory effect of FBE/F1 on adipocyte triglyceride accumulation was attributed to total phenolic (F2) and chlorogenic acid enriched (F3-2) fractions that both inhibited by 75%. Pure compounds catechol (CAT) and chlorogenic acid (CA) also inhibited adipogenesis by 70%. Treatment with NBE, F1, F3-2, CAT and CA decreased p-AKT, whereas p-AMPK tended to increase with F1. The expression of SREBP1-c was not significantly modulated. In contrast, PPARγ decreased in all experimental groups that inhibited adipogenesis.
These results demonstrate that fermented blueberry extract contains compounds with anti-adipogenic activity, which can serve to standardize nutraceutical preparations from fermented blueberry juice and to develop novel compounds with anti-obesity properties.
肥胖症和2型糖尿病在全球已呈流行态势。野生矮丛蓝莓（Vaccinium angustifolium Aiton）是北美原住民传统药典中的一种植物，具有抗糖尿病潜力，尤其是与 vaccinii 沙雷氏菌发酵后。
采用植物化学分离方案来鉴定潜在的生物活性化合物，通过高效液相色谱保留时间和紫外可见光谱进行确认。3T3-L1细胞用普通蓝莓提取物（NBE）、发酵蓝莓提取物（FBE/F1）、7个组分和4种纯化合物分化7天。测量甘油三酯含量。通过蛋白质免疫印迹分析对选定的细胞内信号传导成分（p-Akt、p-AMPK）和转录因子（SREBP-1c和PPARγ）进行检测。
FBE/F1对脂肪细胞甘油三酯积累的抑制作用归因于总酚类（F2）和富含绿原酸的组分（F3-2），二者均有75%的抑制率。纯化合物儿茶酚（CAT）和绿原酸（CA）也使脂肪生成减少70%。用NBE、F1、F3-2、CAT和CA处理可降低p-AKT，而p-AMPK则随F1处理有升高趋势。SREBP1-c的表达未受到显著调节。相反，在所有抑制脂肪生成的实验组中PPARγ均降低。
这些结果表明，发酵蓝莓提取物含有具有抗脂肪生成活性的化合物，这有助于规范发酵蓝莓汁的营养保健品制剂，并开发具有抗肥胖特性的新型化合物。
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