Kim Sung Min, Park Eun-Jung, Kim Jong-Yeon, Choi Jihee, Lee Hae-Jeung
Department of Food and Nutrition, College of BioNano Technology, Gachon University, Seongnam, Gyeonggi-do 13120, Korea.
Institute for Aging and Clinical Nutrition Research, Gachon University, Seongnam, Gyeonggi-do 13120, Korea.
Life (Basel). 2020 Nov 19;10(11):293. doi: 10.3390/life10110293.
Inflammation is a protective response of the innate immune system. However, aberrant inflammatory responses lead to various diseases. Lotus root, the edible rhizome of , is a popular traditional herbal medicine in East Asia. In a previous study, we reported that fermented lotus root (FLR) alleviated ethanol/HCl-induced gastric ulcers in rats by modulating inflammation-related genes. However, the mechanisms underlying the anti-inflammatory effects of FLR and its major constituent, linoleic acid (LA), are still largely unknown. In this study, we investigated the anti-inflammatory effects of FLR and LA on lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 murine macrophages. We found that FLR inhibited LPS-induced expression of inflammatory mediators through down-regulation of NF-κB activity. Similarly, LA also attenuated LPS-induced inflammatory responses and reduced LPS-induced phosphorylation of proteins associated with NF-κB signaling, such as ERK, JNK, and p38. Overall, our results suggested that FLR and LA may effectively ameliorate inflammatory diseases.
炎症是先天性免疫系统的一种保护反应。然而，异常的炎症反应会导致各种疾病。莲藕，即莲的可食用根茎，是东亚一种广受欢迎的传统草药。在之前的一项研究中，我们报道发酵莲藕（FLR）通过调节炎症相关基因减轻了乙醇/盐酸诱导的大鼠胃溃疡。然而，FLR及其主要成分亚油酸（LA）的抗炎作用机制仍 largely unknown。在本研究中，我们研究了FLR和LA对脂多糖（LPS）诱导的RAW 264.7小鼠巨噬细胞炎症的抗炎作用。我们发现FLR通过下调NF-κB活性抑制LPS诱导的炎症介质表达。同样，LA也减弱了LPS诱导的炎症反应，并降低了LPS诱导的与NF-κB信号相关蛋白（如ERK、JNK和p38）的磷酸化。总体而言，我们的结果表明FLR和LA可能有效改善炎症性疾病。 （注：“largely unknown”这里翻译为“很大程度上未知”，感觉表述不太准确，可能原文有误，推测可能是“largely unkown”，若按此推测应翻译为“基本上未知” ）
