López G, Cañas-Duarte S J, Pinzón-Velasco A M, Vega-Vela N E, Rodríguez M, Restrepo S, Baena S
Departamento de Biología, Pontificia Universidad Javeriana, POB 56710, Bogotá D.C., Colombia.
Biological Sciences Department, Universidad de los Andes, Cra 1 No. 18A - 12, Bogotá D.C., Colombia; Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
Syst Appl Microbiol. 2017 Mar;40(2):86-91. doi: 10.1016/j.syapm.2016.11.006. Epub 2016 Dec 16.
Strain USBA-019, an anaerobic and thermophilic strain, was identified as a new member of the genus Thermoanaerobacterium. USBA-019 cells are gram-positive, strictly anaerobic, thermophilic, chemoorganotrophic, moderately acidophilic, non-motile, endospore-forming, slightly curved, and rod-shaped. Cells measure 0.4×3.0-7.0μm. Optimal growth occurs at 50-55°C (35-65°C). Optimum pH is 5.0-5.5 (4.0-8.5). Thiosulfate, elemental sulfur and nitrate were utilized as electron acceptors. Fermentation of glucose, lactose, cellobiose, galactose, arabinose, xylose, starch and xylan primarily produced acetate and butyrate. Xylan, starch and cellobiose produced ethanol and starch, cellobiose, galactose, arabinose and mannose produced lactic acid. Phylogenetic analyses based on 16S rRNA gene sequence comparison and genomic relatedness indices show the close relation of USBA-019 to Thermoanaerobacterium thermostercoris and Thermoanaerobacterium aotearoense (similarity value: 99%). Hybridization of USBA-019, Th. thermostercoris DSM22141 and Th. aotearoense DMS10170 found DNA-DNA relatedness of 33.2% and 18.2%, respectively. Based on phenotypic, chemotaxonomic and phylogenetic evidence, along with low identity at whole genome level, USBA-019 is a novel species of the genus Thermoanaerobacterium which we propose to name Thermoanaerobacterium butyriciformans sp. nov. The type strain is USBA-019 (=CMPUJ U-019=DSM 101588).
菌株USBA - 019是一种厌氧嗜热菌株，被鉴定为嗜热厌氧杆菌属的一个新成员。USBA - 019细胞革兰氏阳性，严格厌氧，嗜热，化能有机营养型，中度嗜酸，不运动，产芽孢，稍弯曲，呈杆状。细胞大小为0.4×3.0 - 7.0μm。在50 - 55°C（35 - 65°C）时生长最佳。最适pH为5.0 - 5.5（4.0 - 8.5）。硫代硫酸盐、元素硫和硝酸盐被用作电子受体。葡萄糖、乳糖、纤维二糖、半乳糖、阿拉伯糖、木糖、淀粉和木聚糖的发酵主要产生乙酸盐和丁酸盐。木聚糖、淀粉和纤维二糖产生乙醇，淀粉、纤维二糖、半乳糖、阿拉伯糖和甘露糖产生乳酸。基于16S rRNA基因序列比较和基因组相关性指数的系统发育分析表明，USBA - 019与嗜热粪嗜热厌氧杆菌和奥塔哥嗜热厌氧杆菌关系密切（相似性值：99%）。USBA - 0与嗜热粪嗜热厌氧杆菌DSM22141和奥塔哥嗜热厌氧杆菌DMS10170的杂交发现DNA - DNA相关性分别为33.2%和18.2%。基于表型、化学分类学和系统发育证据，以及全基因组水平的低同一性，USBA - 019是嗜热厌氧杆菌属的一个新物种，我们提议将其命名为丁酸嗜热厌氧杆菌新种。模式菌株为USBA - 019（=CMPUJ U - 019=DSM 101588）。
