Ahmed Kawther K, Geary Sean M, Salem Aliasger K
Division of Pharmaceutics and Translational Therapeutics, University of Iowa College of Pharmacy, Iowa City, IA, USA.
Division of Pharmaceutics and Translational Therapeutics, University of Iowa College of Pharmacy, Iowa City, IA, USA.
J Control Release. 2017 Feb 28;248:1-9. doi: 10.1016/j.jconrel.2016.12.036. Epub 2017 Jan 3.
Cell surface engineering is an expanding field and whilst extensive research has been performed decorating cell surfaces with biomolecules, the engineering of cell surfaces with particles has been a largely unexploited area. This study reports on the assembly of cell-particle hybrids where irradiated tumor cells were surface engineered with adjuvant-loaded, biodegradable, biocompatible, polymeric particles, with the aim of generating a construct capable of functioning as a therapeutic cancer vaccine. Successfully assembled cell-particle hybrids presented here comprised either melanoma cells or prostate cancer cells stably adorned with Toll-like receptor-9 ligand-loaded particles using streptavidin-biotin cross-linking. Both cell-particle assemblies were tested in vivo for their potential as therapeutic cancer vaccines yielding promising therapeutic results for the prostate cancer model. The ramifications of results obtained for both tumor models are openly discussed.
细胞表面工程是一个不断发展的领域，虽然已经进行了大量关于用生物分子修饰细胞表面的研究，但用颗粒对细胞表面进行工程改造在很大程度上仍是一个未被开发的领域。本研究报告了细胞-颗粒杂合体的组装，其中经辐照的肿瘤细胞通过负载佐剂、可生物降解、生物相容性的聚合物颗粒进行表面工程改造，目的是构建一种能够作为治疗性癌症疫苗发挥作用的结构。这里成功组装的细胞-颗粒杂合体包括黑色素瘤细胞或前列腺癌细胞，它们通过链霉亲和素-生物素交联稳定地装饰有负载Toll样受体-9配体的颗粒。两种细胞-颗粒组装体均在体内测试了其作为治疗性癌症疫苗的潜力，对前列腺癌模型产生了有前景的治疗结果。文中公开讨论了两种肿瘤模型所获结果的影响。
