Sun Wu-Sheng, Chun Ju-Lan, Kim Dong-Hwan, Ahn Jin-Seop, Kim Min-Kyu, Hwang In-Sul, Kwon Dae-Jin, Hwang Seongsoo, Lee Jeong-Woong
Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea.
Department of Animal Science and Biotechnology, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, Korea.
J Vet Sci. 2017 Dec 31;18(4):531-540. doi: 10.4142/jvs.2017.18.4.531.
Ribosomal protein L21 (RPL21) is a structural component of the 60S subunit of the eukaryotic ribosome. This protein has an important role in protein synthesis and the occurrence of hereditary diseases. Pig is a common laboratory model, however, to the best of our knowledge, its gene has not been cloned to date. In this study, we cloned and identified the full-length sequence of the pig gene for the first time. In addition, we examined its expression pattern and function by using overexpression or knockdown approaches. As a result, we obtained a 604 bp segment that contains a 483 bp open reading frame encoding 160 amino acids. The pig gene is located in the "+" strand of chromosome 11, which spans 2167 bp from 4199792 to 4201958. Pig protein has nine strands and two helices in its secondary structure. Pig is predominantly expressed in ovary and lung, at lower levels in kidney, small intestine, and skin, and at the lowest levels in heart and liver. Furthermore, expression is closely connected with cell proliferation and cell cycle arrest. The results are intended to provide useful information for the further study of pig .
核糖体蛋白L21（RPL21）是真核生物核糖体60S亚基的结构组成部分。该蛋白在蛋白质合成和遗传性疾病的发生中起重要作用。猪是一种常见的实验模型，然而，据我们所知，其基因迄今尚未被克隆。在本研究中，我们首次克隆并鉴定了猪该基因的全长序列。此外，我们通过过表达或敲低方法研究了其表达模式和功能。结果，我们获得了一个604 bp的片段，其中包含一个483 bp的开放阅读框，编码160个氨基酸。猪该基因位于11号染色体的“+”链上，跨度为2167 bp，从4199792到4201958。猪该蛋白的二级结构有九条链和两个螺旋。猪该蛋白主要在卵巢和肺中表达，在肾脏、小肠和皮肤中表达水平较低，在心脏和肝脏中表达水平最低。此外，该蛋白的表达与细胞增殖和细胞周期停滞密切相关。这些结果旨在为猪该蛋白的进一步研究提供有用信息。
