Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Department of Medical Biology, Medical School, Pecs University, Pecs, Hungary.
Sci Rep. 2017 Jan 6;7:39927. doi: 10.1038/srep39927.
Multiple pregnancy is a risk for prematurity and preterm birth. The goal of assisted reproduction is to achieve a single pregnancy, by transferring a single embryo. This requires improved methods to identify the competent embryo. Here, we describe such a test, based on flow cytometric determination of the nucleic acid (PI+) containing extracellular vesicle (EV) count in day 5 embryo culture media. 88 women undergoing IVF were included in the study. More than 1 embryos were transferred to most patients. In 58 women, the transfer resulted in clinical pregnancy, whereas in 30 women in implantation failure. In 112 culture media of embryos from the "clinical pregnancy" group, the number of PI+ EVs was significantly lower than in those of 49 embryos, from the "implantation failure" group. In 14 women, transfer of a single embryo resulted in a singleton pregnancy, or, transfer of two embryos in twin pregnancy. The culture media of 19 out of the 20 "confirmed competent" embryos contained a lower level of PI+ EVs than the cut off level, suggesting that the competent embryo can indeed be identified by low PI+ EV counts. We developed a noninvasive, simple, inexpensive, quick test, which identifies the embryos that are most likely to implant.
多胎妊娠是早产和早产的风险。辅助生殖的目标是通过转移单个胚胎来实现单胎妊娠。这需要改进方法来识别有能力的胚胎。在这里，我们描述了一种基于第 5 天胚胎培养物中核酸（PI+）含量的流式细胞术来确定含有细胞外囊泡（EV）计数的测试。研究纳入了 88 名接受 IVF 的女性。大多数患者都移植了超过 1 个胚胎。在 58 名女性中，转移导致临床妊娠，而在 30 名女性中导致着床失败。在 58 名“临床妊娠”组胚胎的 112 个培养物中，PI+EV 的数量明显低于 49 个胚胎的培养物，来自“着床失败”组。在 14 名妇女中，转移单个胚胎导致单胎妊娠，或转移两个胚胎导致双胞胎妊娠。20 个“确认有能力”胚胎中的 19 个胚胎的培养物中的 PI+EV 含量低于截止值，这表明通过低 PI+EV 计数确实可以识别有能力的胚胎。我们开发了一种非侵入性、简单、廉价、快速的测试方法，可以识别最有可能着床的胚胎。
