Wang Chenghong, Qiu Jiguo, Yang Youjian, Zheng Jinwei, He Jian, Li Shunpeng
College of Basic Medical Science, Jiujiang University, Jiujiang, Jiangxi, 332000, China.
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
Biotechnol Lett. 2017 Apr;39(4):553-560. doi: 10.1007/s10529-016-2276-z. Epub 2017 Jan 5.
To identify and characterize a novel aryloxyphenoxypropionate (AOPP) herbicide-hydrolyzing carboxylesterase from Aquamicrobium sp. FPB-1.
A carboxylesterase gene, fpbH, was cloned from Aquamicrobium sp. FPB-1. The gene is 798 bp long and encodes a protein of 265 amino acids. FpbH is smaller than previously reported AOPP herbicide-hydrolyzing carboxylesterases and shares only 21-35% sequence identity with them. FpbH was expressed in Escherichia coli BL21(DE3) and the product was purified by Ni-NTA affinity chromatography. The purified FpbH hydrolyzed a wide range of AOPP herbicides with catalytic efficiency in the order: haloxyfop-P-methyl > diclofop-methyl > fenoxaprop-P-ethyl > quizalofop-P-ethyl > fluazifop-P-butyl > cyhalofop-butyl. The optimal temperature and pH for FpbH activity were 37 °C and 7, respectively.
FpbH is a novel AOPP herbicide-hydrolyzing carboxylesterase; it is a good candidate for mechanistic study of AOPP herbicide-hydrolyzing carboxylesterases and for bioremediation of AOPP herbicide-contaminated environments.
从水生微生物菌株FPB - 1中鉴定并表征一种新型的芳氧基苯氧基丙酸酯（AOPP）除草剂水解羧酯酶。
从水生微生物菌株FPB - 1中克隆出一个羧酯酶基因fpbH。该基因长度为798 bp，编码一个含265个氨基酸的蛋白质。FpbH比先前报道的AOPP除草剂水解羧酯酶小，与它们的序列同一性仅为21 - 35%。FpbH在大肠杆菌BL21(DE3)中表达，产物通过镍 - 次氮基三乙酸（Ni - NTA）亲和层析纯化。纯化后的FpbH能水解多种AOPP除草剂，催化效率顺序为：精稳杀得>禾草灵>噁唑禾草灵>喹禾灵>高效盖草能>氰氟草酯。FpbH活性的最适温度和pH分别为37℃和7。
FpbH是一种新型的AOPP除草剂水解羧酯酶；它是AOPP除草剂水解羧酯酶机理研究以及AOPP除草剂污染环境生物修复的良好候选者。
