Tang Huiling, Zhang XiaoRan, Duan Yajuan, Huang Yan
Department of Scientific Research Office, Jiangsu Food and Pharmaceutical Science College, Huai'an 223003, People's Republic of China.
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
Acta Crystallogr F Struct Biol Commun. 2025 Jan 1;81(Pt 1):30-34. doi: 10.1107/S2053230X24012275.
Fenoxaprop-P-ethyl (FE) is widely applied as a post-emergence aryloxyphenoxy propionate (AOPP) herbicide in agriculture. A novel FE hydrolase esterase from Acinetobacter sp. DL-2 (AfeH) was identified which belongs to the family IV carboxylesterases and shows less than 30% identity to other reported homologues with known structure. In order to understand the catalytic mechanism, recombinant AfeH was prepared in Escherichia coli and crystallized using the sitting-drop vapor-diffusion method. X-ray diffraction data were collected to 1.9 Å resolution. The crystal belonged to space group P121, with unit-cell parameters a = 84.27, b = 46.74, c = 258.68 Å. The Matthews coefficient (V) was calculated to be 2.43 Å Da, which corresponds to a solvent content of 49.4%, suggesting the presence of three monomers in the crystallographic asymmetric unit. The crystal was assessed to be suitable for further structural determination, which is currently in progress.
精恶唑禾草灵（FE）作为一种芽后芳氧苯氧丙酸酯（AOPP）类除草剂在农业中被广泛应用。从不动杆菌属DL-2菌株中鉴定出一种新型的FE水解酶酯酶（AfeH），它属于IV型羧酸酯酶家族，与其他已报道的具有已知结构的同源物的同一性低于30%。为了了解其催化机制，在大肠杆菌中制备了重组AfeH，并采用坐滴气相扩散法进行结晶。收集到了分辨率为1.9 Å的X射线衍射数据。该晶体属于空间群P121，晶胞参数a = 84.27、b = 46.74、c = 258.68 Å。计算得到的马修斯系数（V）为2.43 Å Da，对应于49.4%的溶剂含量，表明在晶体学不对称单元中存在三个单体。该晶体被评估适合进行进一步的结构测定，目前相关工作正在进行中。
