Stanovova M V, Erokhov P A, Gornostaev N G, Mikhailov V S, Lyupina Yu V
Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Moscow, Russia.
Dokl Biochem Biophys. 2016 Nov;471(1):428-430. doi: 10.1134/S1607672916060132. Epub 2017 Jan 6.
We investigated functioning of proteasomes and chaperones in Arenicola marina coelomocytes in conditions of lipopolysaccharide-induced inflammation. We observed the increase of chymotrypsin-like proteasome activity in coelomocytes 1 h after induction. Amount of proteasome subunits alpha- and beta-5 types increased as well. We also detected appearance of a new form of Hsp70 chaperone in infected coelomocytes. Our results allow us to consider the changes in proteasome structure and induction of chaperones as principle mechanisms in stress adaptation and defensive reactions development in annelids.
我们研究了在脂多糖诱导的炎症条件下，沙蠋体腔细胞中蛋白酶体和伴侣蛋白的功能。我们观察到诱导后1小时，体腔细胞中类胰凝乳蛋白酶样蛋白酶体活性增加。蛋白酶体α-5和β-5型亚基的数量也增加了。我们还在受感染的体腔细胞中检测到一种新形式的热休克蛋白70伴侣蛋白的出现。我们的结果使我们能够将蛋白酶体结构的变化和伴侣蛋白的诱导视为环节动物应激适应和防御反应发展的主要机制。
