Zhang Lijiao, Ren Ting, Wang Zechun, Wang Ruiyong, Chang Junbiao
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China.
J Mol Recognit. 2017 Jun;30(6). doi: 10.1002/jmr.2606. Epub 2017 Jan 6.
This study aims to investigate the interaction between 3 flavonoids (quercetin, apigenin, and naringenin) and fat mass and obesity-associated protein by fluorescence, ultraviolet-visible absorption spectroscopy, and molecular modeling. Results indicate that the intrinsic fluorescence of fat mass and obesity-associated protein can be quenched by the 3 flavonoids through a static quenching procedure. Thermodynamic analysis and molecular modeling results suggest that hydrophobic interaction and hydrogen bond forces play the major roles in the binding process. Moreover, results also show that the rank order of quenching constant and binding constant is quercetin > apigenin > naringenin.
本研究旨在通过荧光、紫外可见吸收光谱和分子模拟研究3种黄酮类化合物（槲皮素、芹菜素和柚皮素）与脂肪量和肥胖相关蛋白之间的相互作用。结果表明，3种黄酮类化合物可通过静态猝灭过程猝灭脂肪量和肥胖相关蛋白的内在荧光。热力学分析和分子模拟结果表明，疏水相互作用和氢键力在结合过程中起主要作用。此外，结果还表明，猝灭常数和结合常数的顺序为槲皮素>芹菜素>柚皮素。
