Liu Shujuan, Zhou Na, Chen Zejing, Wei Huanjie, Zhu Yana, Guo Song, Zhao Qiang
Opt Lett. 2017 Jan 1;42(1):13-16. doi: 10.1364/OL.42.000013.
A reducing intracellular environment is necessary for living cells. Here a redox-sensitive phosphorescent probe Ir-NO has been developed for evaluating the redox environment in living cells. Upon addition of reducing molecules, such as glutathione and ascorbic acid, the phosphorescent intensity of the probe is turned on, and the emission lifetime is elongated evidently. Furthermore, this probe has been used for optical imaging of the intracellular reducing environment by utilizing confocal laser scanning microscopy and phosphorescence lifetime imaging microscopy.
还原性细胞内环境对活细胞而言是必要的。在此，一种对氧化还原敏感的磷光探针Ir-NO已被开发用于评估活细胞内的氧化还原环境。加入还原性分子，如谷胱甘肽和抗坏血酸后，探针的磷光强度开启，且发射寿命明显延长。此外，该探针已通过共聚焦激光扫描显微镜和磷光寿命成像显微镜用于细胞内还原环境的光学成像。
