高荧光量子产率线粒体靶向近红外荧光探针用于活细胞内和体内天然次溴酸的成像。
High-Quantum-Yield Mitochondria-Targeting Near-Infrared Fluorescent Probe for Imaging Native Hypobromous Acid in Living Cells and in Vivo.
机构信息
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institute of Molecular and Nano Science, Shandong Normal University , Jinan, Shandong 250014, P. R. China.
出版信息
Anal Chem. 2017 Feb 7;89(3):1787-1792. doi: 10.1021/acs.analchem.6b04094. Epub 2017 Jan 19.
The discovery that hypobromous acid (HOBr) can regulate the activity of collagen IV has attracted great attention. However, HOBr as an important reactive small molecule has hardly ever been studied using a detection method suitable for organisms. Herein, a high-quantum-yield mitochondria-targeting near-infrared (NIR) fluorescent probe for HOBr, RhSN-mito, was designed. RhSN-mito was easily obtained by the Suzuki cross-coupling reaction. The test results show that RhSN-mito can rapidly respond to HOBr with ultrasensitivity and high selectivity. The achievement of ultrasensitivity lies in the high signal-to-noise ratio and the highest fluorescence quantum yield of the reaction product (Φ = 0.68) in the near-infrared region, as far as we know. RhSN-mito is successfully applied to image native HOBr in mitochondria of HepG2 cells and zebrafish. Thus, RhSN-mito is a powerful tool for detecting native HOBr in vivo and is expected to provide a method to further study the physiological and pathological functions related to HOBr.
次溴酸（HOBr）能够调节 IV 型胶原活性这一发现引起了极大关注。然而，HOBr 作为一种重要的反应性小分子，几乎从未使用适合生物体的检测方法进行过研究。在此，设计了一种用于检测 HOBr 的高量子产率线粒体靶向近红外（NIR）荧光探针 RhSN-mito。通过 Suzuki 交叉偶联反应很容易得到 RhSN-mito。实验结果表明，RhSN-mito 能够快速、灵敏、高选择性地响应 HOBr。实现超灵敏性的原因在于反应产物在近红外区域具有高信噪比和最高的荧光量子产率（Φ=0.68），据我们所知。RhSN-mito 成功地应用于 HepG2 细胞和斑马鱼线粒体中内源性 HOBr 的成像。因此，RhSN-mito 是一种用于在体内检测内源性 HOBr 的有力工具，有望为进一步研究与 HOBr 相关的生理和病理功能提供一种方法。
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