Ku Hui-Chun, Lee Shih-Yi, Wu Yuan-Kun Aden, Yang Kai-Chien, Su Ming-Jai
Graduate Institute of Pharmacology, College of Medicine, National Taiwan University.
Division of Pulmonary and Critical Care Medicine, Mackay Memorial Hospital; Mackay Medicine, Nursing and Management College.
J Vis Exp. 2016 Dec 2(118):54818. doi: 10.3791/54818.
Heart failure is one of the leading causes of death worldwide. It is a complex clinical syndromethat includes fatigue, dyspnea, exercise intolerance, and fluid retention. Changes in myocardial structure, electrical conduction, and energy metabolism develop with heart failure, leading to contractile dysfunction, increased risk of arrhythmias, and sudden death. Hypertensive heart disease is one of the key contributing factors of cardiac remodeling associated with heart failure. The most commonly-used animal model mimicking hypertensive heart disease is created via surgical interventions, such as by narrowing the aorta. Abdominal aortic constriction is a useful experimental technique to induce a pressure overload, which leads to heart failure. The surgery can be easily performed, without the need for chest opening or mechanical ventilation. Abdominal aortic constriction-induced cardiac pathology progresses gradually, making this model relevant to clinical hypertensive heart failure. Cardiac injury and remodeling can be observed 10 weeks after the surgery. The method described here provides a simple and effective approach to produce a hypertensive heart disease animal model that is suitable for studying disease mechanisms and for testing novel therapeutics.
心力衰竭是全球主要死因之一。它是一种复杂的临床综合征，包括疲劳、呼吸困难、运动耐量下降和液体潴留。心力衰竭时会出现心肌结构、电传导和能量代谢的变化，导致收缩功能障碍、心律失常风险增加和猝死。高血压性心脏病是与心力衰竭相关的心脏重塑的关键促成因素之一。最常用的模拟高血压性心脏病的动物模型是通过手术干预创建的，例如通过缩窄主动脉。腹主动脉缩窄是一种诱导压力超负荷从而导致心力衰竭的有用实验技术。该手术操作简便，无需开胸或机械通气。腹主动脉缩窄诱导的心脏病变会逐渐进展，使该模型与临床高血压性心力衰竭相关。术后10周可观察到心脏损伤和重塑。这里描述的方法提供了一种简单有效的方法来制作适合研究疾病机制和测试新型疗法的高血压性心脏病动物模型。
