Cui X X
Cancer Institute, Chinese Academy of Medical Sciences, Beijing.
Zhonghua Zhong Liu Za Zhi. 1989 Mar;11(2):101-3.
N-Nitrosothiazolidine 4-carboxylic acid (NTCA), a nonvolatile N-Nitroso compound containing sulfur was first found in the urine of normal subjects in Lin-xian county, a high risk area of esophageal cancer. The content of NTCA in the urine of the general population of Lin-xian is higher than that in Fan-xian county, a low risk area of esophageal cancer. NTCA could induce mutation in V79 cells. The results showed that NIH 3T3 cells could be induced to undergo malignant transformation by NTCA. If NIH 3T3 cells were cultured in soft agar medium, transformed cells could grow progressively to form colonies and became anchorage-independent. These transformed cells were tumorigenic in nude mice. The above results indicate that NTCA formed in vivo, being one of the risk factors of esophageal cancer in Lin-xian county, is a potential carcinogen. This investigation also demonstrates that the N-Nitroso compounds are closely related to esophageal cancer.
N-亚硝基硫代乙内酰脲-4-羧酸（NTCA）是一种含硫的非挥发性N-亚硝基化合物，最初在食管癌高发区林县正常人群的尿液中被发现。林县普通人群尿液中NTCA的含量高于食管癌低发区范县人群尿液中NTCA的含量。NTCA可诱导V79细胞发生突变。结果表明，NTCA可诱导NIH 3T3细胞发生恶性转化。若将NIH 3T3细胞培养于软琼脂培养基中，转化细胞可逐渐生长形成集落，并变得不依赖贴壁生长。这些转化细胞在裸鼠体内具有致瘤性。上述结果表明，体内形成的NTCA是林县食管癌的危险因素之一，是一种潜在致癌物。本研究还表明，N-亚硝基化合物与食管癌密切相关。
