人类多形核白细胞葡萄糖转运测量的临床应用

Clinical application of measurement of glucose transport in human polymorphonuclear leukocytes.

作者信息

Okuno Y, Morii H

机构信息

Second Department of Internal Medicine, Osaka City University Medical School, Japan.

出版信息

Diabetes Res Clin Pract. 1989;7 Suppl 1:S5-9. doi: 10.1016/0168-8227(89)90081-8.

DOI:10.1016/0168-8227(89)90081-8

PMID:2806056

Abstract

Transport of the non-metabolizable hexose analogue 3-O-methyl-D-glucose (3OMG) was measured at 37 degrees C, pH 7.4, in human polymorphonuclear leukocytes (PMNLs) obtained from 15 ml of fresh venous blood. In the study, 0.05 mM of 3OMG was equilibrated with a half-time of about 10 s, and the rate constant was 0.074/s in PMNLs from a healthy subject (male, 38 years). The coefficient of variation of values assayed on different days was about 7% and the intra-assay variation was about 2%. The transport rate did not differ between the two sexes or between subjects of different ages (23-63 years), but was significantly higher in 23 patients with non-insulin-dependent diabetes than in 29 normal controls (13.3 +/- 3.7 vs. 10.4 +/- 2.5 fl/cell.s, mean +/- SD). In conclusion, the measurement of transport of 3OMG in PMNLs may be useful for the study of glucose transport in clinical investigations.

摘要

在37摄氏度、pH值为7.4的条件下，对从15毫升新鲜静脉血中获取的人多形核白细胞（PMNLs）的不可代谢己糖类似物3 - O - 甲基 - D - 葡萄糖（3OMG）的转运进行了测量。在该研究中，0.05 mM的3OMG在约10秒的半衰期达到平衡，来自一名健康受试者（男性，38岁）的PMNLs中的速率常数为0.074/秒。不同日期测定值的变异系数约为7%，测定内变异约为2%。转运速率在两性之间或不同年龄（23 - 63岁）的受试者之间没有差异，但在23例非胰岛素依赖型糖尿病患者中显著高于29例正常对照（13.3±3.7对10.4±2.5飞升/细胞·秒，平均值±标准差）。总之，在临床研究中，测量PMNLs中3OMG的转运可能有助于葡萄糖转运的研究。