Seemann Frauke, Jeong Chang-Bum, Zhang Ge, Wan Miles Teng, Guo Baosheng, Peterson Drew Ryan, Lee Jae-Seong, Au Doris Wai-Ting
State Key Laboratory in Marine Pollution, Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Hong Kong.
Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
Aquat Toxicol. 2017 Feb;183:127-134. doi: 10.1016/j.aquatox.2016.12.018. Epub 2016 Dec 23.
Benzo[a]pyrene (BaP) at an environmentally relevant concentration (1μg/L) has previously been shown to affect bone development in a transgenerational manner in F3 medaka (Oryzias latipes) larvae (17dph). Here, we provide novel histomorphometric data demonstrating that the impaired bone formation at an early life stage is not recoverable and can result in a persistent transgenerational impairment of bone metabolism in F3 adult fish. A decrease in bone thickness and the occurrence of microcracks in ancestrally BaP-treated adult male fish (F3) were revealed by MicroCt measurement and histopathological analysis. The expression of twenty conserved bone miRNAs were screened in medaka and their relative expression (in the F3 ancestral BaP treatment vs the F3 control fish) were determined by quantitative real-time PCR. Attempt was made to link bone miRNA expression with the potential target bone mRNA expression in medaka. Five functional pairs of mRNA/miRNA were identified (Osx/miR-214, Col2a1b/miR-29b, Runx2/miR-204, Sox9b/miR-199a-3p, APC/miR-27b). Unique knowledge of bone-related miRNA expression in medaka in response to ancestral BaP-exposure in the F3 generation is presented. From the ecological risk assessment perspective, BaP needs to be regarded as a transgenerational skeletal toxicant which exerts a far-reaching impact on fish survival and fitness. Given that the underlying mechanisms of cartilage/bone formation are conserved between medaka and mammals, the results may also shed light on the potential transgenerational effect of BaP on skeletal disorders in mammals/humans.
先前的研究表明，环境相关浓度（1μg/L）的苯并[a]芘（BaP）会以跨代方式影响F3青鳉（Oryzias latipes）幼虫（17日龄）的骨骼发育。在此，我们提供了新的组织形态计量学数据，表明生命早期阶段的骨形成受损是不可恢复的，并且会导致F3成年鱼的骨代谢持续出现跨代损伤。通过MicroCt测量和组织病理学分析发现，经BaP处理的亲代成年雄鱼（F3）的骨厚度降低且出现微裂纹。在青鳉中筛选了20种保守的骨miRNA的表达，并通过定量实时PCR测定了它们的相对表达（F3亲代BaP处理组与F3对照鱼相比）。我们试图将青鳉中骨miRNA的表达与潜在的靶标骨mRNA表达联系起来。鉴定出了五对功能性的mRNA/miRNA（Osx/miR - 214、Col2a1b/miR - 29b、Runx2/miR - 204、Sox9b/miR - 199a - 3p、APC/miR - 27b）。本文展示了F3代青鳉对亲代BaP暴露的响应中与骨相关的miRNA表达的独特知识。从生态风险评估的角度来看，BaP应被视为一种跨代骨骼毒物它对鱼类的生存和健康有着深远的影响。鉴于青鳉和哺乳动物之间软骨/骨形成的潜在机制是保守的，这些结果也可能为BaP对哺乳动物/人类骨骼疾病的潜在跨代影响提供线索。
