Department of Creative Engineering, National Institute of Technology, Ariake College, 150 Higashi-Hagio, Omuta, Fukuoka, 836-8585, Japan.
Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, 790-8566, Japan.
Chemosphere. 2020 Mar;242:125243. doi: 10.1016/j.chemosphere.2019.125243. Epub 2019 Oct 31.
Polycyclic aromatic hydrocarbons (PAHs), such as benzo[a]pyrene (BaP), are widely distributed in air, water, and sediments; however, limited data are available regarding their potential adverse effects on the early life stages of fish. In this study, we evaluated the embryonic teratogenicity and developmental toxicity of BaP in Japanese medaka (Oryzias latipes) using a nanosecond pulsed electric field (nsPEF) technique and predicted their molecular mechanisms via transcriptome analysis. The gas chromatography/mass spectrometry analyses revealed that the BaP was efficiently incorporated into the embryos by nsPEF treatment. The embryos incorporating BaP presented typical teratogenic and developmental effects, such as cardiovascular abnormalities, developmental abnormalities, and curvature of backbone. DNA microarray analysis revealed several unique upregulated genes, such as those involved in cardiovascular diseases, various cellular processes, and neural development. Furthermore, the gene set enrichment and network analyses found several genes and hub proteins involved in the developmental effects of BaP on the embryos. These findings suggest a potential mechanism of teratogenicity and developmental toxicity caused by exposure to BaP. The nsPEF and transcriptome analyses in combination can be effective for evaluating the potential effects of chemical substances on medaka embryos.
多环芳烃（PAHs），如苯并[a]芘（BaP），广泛分布于空气、水和沉积物中；然而，关于它们对鱼类早期生活阶段的潜在不良影响的数据有限。在这项研究中，我们使用纳秒脉冲电场（nsPEF）技术评估了 BaP 对日本青鳉（Oryzias latipes）胚胎的致畸性和发育毒性，并通过转录组分析预测了它们的分子机制。气相色谱/质谱分析表明，BaP 通过 nsPEF 处理有效地被胚胎吸收。吸收了 BaP 的胚胎表现出典型的致畸和发育异常，如心血管异常、发育异常和脊柱弯曲。DNA 微阵列分析发现了几个独特的上调基因，如参与心血管疾病、各种细胞过程和神经发育的基因。此外，基因集富集和网络分析发现了几个与 BaP 对胚胎发育影响相关的基因和枢纽蛋白。这些发现表明了暴露于 BaP 引起致畸性和发育毒性的潜在机制。nsPEF 和转录组分析的结合可有效评估化学物质对青鳉胚胎的潜在影响。
