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在甘蔗糖蜜中培养微藻:选择、生长和生物化学特性分析。

Microalgae cultivation in sugarcane vinasse: Selection, growth and biochemical characterization.

机构信息

Embrapa Agroenergy, Brasília/DF, Brazil; Universidade Federal da Bahia, Vitória da Conquista/BA, Brazil.

Embrapa Agroenergy, Brasília/DF, Brazil; Universidade Federal do Tocantins, Gurupi/TO, Brazil.

出版信息

Bioresour Technol. 2017 Mar;228:133-140. doi: 10.1016/j.biortech.2016.12.075. Epub 2016 Dec 23.

Abstract

Sugarcane ethanol is produced at large scale generating wastes that could be used for microalgae biomass production in a biorefinery strategy. In this study, forty microalgae strains were screened for growth in sugarcane vinasse at different concentrations. Two microalgae strains, Micractinium sp. Embrapa|LBA32 and C. biconvexa Embrapa|LBA40, presented vigorous growth in a light-dependent manner even in undiluted vinasse under non-axenic conditions. Microalgae strains presented higher biomass productivity in vinasse-based media compared to standard Bold's Basal Medium in cultures performed using 15L airlift flat plate photobioreactors. Chemical composition analyses showed that proteins and carbohydrates comprise the major fractions of algal biomass. Glucose was the main monosaccharide detected, ranging from 46% to 76% of the total carbohydrates content according to the strain and culture media used. This research highlights the potential of using residues derived from ethanol plants to cultivate microalgae for the production of energy and bioproducts.

摘要

甘蔗乙醇是大规模生产的,会产生废物,可以在生物炼制策略中用于生产微藻生物质。在这项研究中,筛选了 40 株微藻菌株,以研究它们在不同浓度的甘蔗废醪液中的生长情况。两种微藻菌株,即 Micractinium sp.Embrapa|LBA32 和 C. biconvexa Embrapa|LBA40,即使在非无菌条件下未经稀释的废醪液中,也能以依赖光照的方式旺盛生长。与在使用 15L 气升式平板光生物反应器进行的培养中使用标准 Bold's Basal Medium 相比,微藻菌株在基于废醪液的培养基中的生物量生产力更高。化学成分分析表明,蛋白质和碳水化合物构成了藻类生物质的主要部分。根据菌株和培养基的不同,葡萄糖是检测到的主要单糖,其含量占总碳水化合物含量的 46%至 76%。这项研究强调了利用乙醇厂的残留物来培养微藻以生产能源和生物制品的潜力。

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