State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Bioresour Technol. 2017 Mar;228:99-105. doi: 10.1016/j.biortech.2016.12.084. Epub 2016 Dec 24.
Transcriptome sequencing and annotation was performed on Haematococcus pluvialis mutant red cells induced with high light under 15% CO to demonstrate why astaxanthin yield of the mutant was 1.7 times higher than that of a wild strain. It was found that 56% of 1947 differentially expressed genes were upregulated in mutant cells. Most significant differences were found in unigenes related to photosynthesis, carotenoid biosynthesis and fatty acid biosynthesis pathways. The pyruvate kinase increased by 3.5-fold in mutant cells. Thus, more pyruvate, which was beneficial to carotenoids and fatty acid biosynthesis, was generated. Phytoene synthase, zeta-carotene desaturase, lycopene beta-cyclase involved in β-carotene biosynthesis in mutant cells were upregulated by 10.4-, 4.4-, and 5.8-fold, respectively. Beta-carotene 3-hydroxylase catalyzing conversion of β-carotene into astaxanthin was upregulated by 18.4-fold. The fatty acid biosynthesis was promoted because of the upregulation of acetyl-CoA synthetase and acetyl-CoA carboxylase, thus increasing astaxanthin esterification and accumulation in mutant cells.
对高光下 15% CO 诱导的雨生红球藻突变红细胞进行转录组测序和注释，以阐明突变体虾青素产量为何比野生株高 1.7 倍。结果发现，1947 个差异表达基因中有 56%在突变细胞中上调。与光合作用、类胡萝卜素生物合成和脂肪酸生物合成途径相关的基因在差异表达基因中差异最显著。突变细胞中的丙酮酸激酶增加了 3.5 倍。因此，生成了更多有利于类胡萝卜素和脂肪酸生物合成的丙酮酸。突变细胞中参与β-胡萝卜素生物合成的八氢番茄红素合酶、ζ-胡萝卜素脱饱和酶、番茄红素 β-环化酶分别上调了 10.4、4.4 和 5.8 倍。催化β-胡萝卜素转化为虾青素的β-胡萝卜素 3-羟化酶上调了 18.4 倍。由于乙酰辅酶 A 合成酶和乙酰辅酶 A 羧化酶的上调，促进了脂肪酸的生物合成，从而增加了突变细胞中虾青素的酯化和积累。
