State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Bioresour Technol. 2017 Jun;233:313-321. doi: 10.1016/j.biortech.2017.02.121. Epub 2017 Feb 28.
To elucidate the mechanism underlying the enhanced growth rate in the Haematococcus pluvialis mutated with Co-γ rays and domesticated with 15% CO, transcriptome sequencing was conducted to clarify the carbon metabolic pathways of mutant cells. The CO fixation rate of mutant cells increased to 2.57gLd under 15% CO due to the enhanced photosynthesis, carbon fixation, glycolysis pathways. The upregulation of PetH, ATPF0A and PetJ related to photosynthetic electron transport, ATP synthase and NADPH generation promoted the photosynthesis. The upregulation of genes related to Calvin cycle and ppdK promoted carbon fixation in both C3 and C4 photosynthetic pathways. The reallocation of carbon was also enhanced under 15% CO. The 19-, 14- and 3.5-fold upregulation of FBA, TPI and PK genes, respectively, remarkably promoted the glycolysis pathways. This accelerated the conversion of photosynthetic carbon to pyruvate, which was an essential precursor for astaxanthin and lipids biosynthesis.
为了阐明经 Co-γ 射线诱变和 15%CO 驯化的雨生红球藻生长速率提高的机制，进行了转录组测序，以阐明突变细胞的碳代谢途径。由于光合作用、碳固定、糖酵解途径的增强，突变细胞在 15%CO 下的 CO 固定率增加到 2.57gLd。与光合电子传递、ATP 合酶和 NADPH 生成相关的 PetH、ATPF0A 和 PetJ 的上调促进了光合作用。与卡尔文循环和 ppdK 相关的基因上调促进了 C3 和 C4 光合作用途径中的碳固定。在 15%CO 下，碳的再分配也得到了增强。FBA、TPI 和 PK 基因的上调分别达到 19 倍、14 倍和 3.5 倍，显著促进了糖酵解途径。这加速了光合碳向丙酮酸的转化，丙酮酸是虾青素和脂质生物合成的必需前体。
