College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, 53 Zhengzhou Road, Qingdao, 266042, China.
School of Life Sciences, Ludong University, 186 Hongqi Middle Road, Yantai, 264025, China.
Mar Biotechnol (NY). 2021 Apr;23(2):255-275. doi: 10.1007/s10126-021-10021-y. Epub 2021 Mar 10.
With atmospheric CO increasing, a large amount of CO is absorbed by oceans and lakes, which changes the carbonate system and affects the survival of aquatic plants, especially microalgae. The main aim of our study was to explore the responses of Chlamydomonas reinhardtii (Chlorophyceae) to elevated CO by combined transcriptome and metabolome analysis under three different scenarios: control (CK, 400 ppm), short-term elevated CO (ST, 1000 ppm), and long-term elevated CO (LT, 1000 ppm). The transcriptomic data showed moderate changes between ST and CK. However, metabolic analysis indicated that fatty acids (FAs) and partial amino acids (AAs) were increased under ST. There was a global downregulation of genes involved in photosynthesis, glycolysis, lipid metabolism, and nitrogen metabolism but increase in the TCA cycle and β-oxidation under LT. Integrated transcriptome and metabolome analyses demonstrated that the nutritional constituents (FAs, AAs) under LT were poor compared with CK, and most genes and metabolites involved in C and N metabolism were significantly downregulated. However, the growth and photosynthesis of cells under LT increased significantly. Thus, C. reinhardtii could form a specific adaptive evolution to elevated CO, affecting future biogeochemical cycles.
随着大气中 CO 的增加，大量的 CO 被海洋和湖泊吸收，这改变了碳酸盐系统，影响了水生植物的生存，尤其是微藻。我们研究的主要目的是通过在三种不同情景下（对照 CK，400 ppm；短期高 CO 处理 ST，1000 ppm；长期高 CO 处理 LT，1000 ppm）的联合转录组和代谢组分析，来探究莱茵衣藻（绿藻门）对高 CO 的响应。转录组数据显示 ST 与 CK 之间的变化不大。然而，代谢分析表明 ST 下脂肪酸（FAs）和部分氨基酸（AAs）增加。LT 下与光合作用、糖酵解、脂质代谢和氮代谢相关的基因普遍下调，但三羧酸循环（TCA 循环）和β-氧化增加。综合转录组和代谢组分析表明，LT 下的营养成分（FAs、AAs）比 CK 差，并且与 C 和 N 代谢相关的大多数基因和代谢物都显著下调。然而，LT 下细胞的生长和光合作用显著增加。因此，莱茵衣藻可以对高 CO 形成特定的适应性进化，影响未来的生物地球化学循环。
